ArticlePLoS biology2012
Haemodynamics-driven developmental pruning of brain vasculature in zebrafish.
Article in PLoS biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 116 papers.
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Who cites it
116 citing papers in PubMed, 265 citations in OpenAlex.
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- Zebrafish model for studying vascular development and vascular-related disease.Frontiers in cell and developmental biology · 2026Review
- Complex relationship among vessel diameter, shear stress and blood pressure controlling vessel pruning during angiogenesis.PLoS computational biology · 2026Article
- Reconsidering the blood-brain barrier: histopathology and microanatomy of cryptococcal CNS infection.Microbiology and molecular biology reviews : MMBR · 2025Review
- NMDA receptors coordinate brain vascular development via neuron-to-endothelial tip cell crosstalk in zebrafish.Nature communications · 2025Article
- Human adult hippocampal neurogenesis is shaped by neuropsychiatric disorders, demographics, and lifestyle-related factors.Cell stem cell · 2025Article
- Temporal dynamics of angiogenesis: the emerging role of mechanoregulated pathways.Biochemical Society transactions · 2025Review
- Progress in recapitulating morphogenesis of blood microvascular structures for microphysiological systems development.Biochemical Society transactions · 2025Article
- Reduction of neuronal activity mediated by blood-vessel regression in the adult brain.Nature communications · 2025Article
- Direct blood flow velocity measurements of zebrafish cerebral vessels with a dual-mode light-sheet microscope.Biomedical optics express · 2025Article
- Reversal of cerebrovascular anomalies in a zebrafish model of vein of Galen aneurysm.Nature cardiovascular research · 2025Article
- Dysfunctional mechanotransduction regulates the progression of PIK3CA-driven vascular malformations.APL bioengineering · 2025Article
- Disruption of Swell1/VRAC function impairs initial hemodynamics and activates compensatory leukotriene signaling in zebrafish circulation development.Frontiers in cell and developmental biology · 2025Article
- Dysfunctional mechanotransduction regulates the progression of PIK3CA-driven vascular malformations.bioRxiv : the preprint server for biology · 2024Article
- The food dye Tartrazine disrupts vascular formation both in zebrafish larvae and in human primary endothelial cells.Scientific reports · 2024Article
- Developmental toxicity and mechanism of dibutyl phthalate on the development of subintestinal vessels in zebrafish.Scientific reports · 2024Article
- Endothelial Piezo1 channel mediates mechano-feedback control of brain blood flow.Nature communications · 2024Article
- Hemodynamic regulation allows stable growth of microvascular networks.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A cell-and-plasma numerical model reveals hemodynamic stress and flow adaptation in zebrafish microvessels after morphological alteration.PLoS computational biology · 2023Article
56 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The brain blood vasculature consists of a highly ramified vessel network that is tailored to meet its physiological functions. How the brain vasculature is formed has long been fascinating biologists. Here we report that the developing vasculature in the zebrafish midbrain undergoes not only angiogenesis but also extensive vessel pruning, which is driven by changes in blood flow. This pruning process shapes the initial exuberant interconnected meshwork into a simplified architecture. Using in vivo long-term serial confocal imaging of the same zebrafish larvae during 1.5-7.5 d post-fertilization, we found that the early formed midbrain vasculature consisted of many vessel loops and higher order segments. Vessel pruning occurred preferentially at loop-forming segments via a process mainly involving lateral migration of endothelial cells (ECs) from pruned to unpruned segments rather than EC apoptosis, leading to gradual reduction in the vasculature complexity with development. Compared to unpruned ones, pruned segments exhibited a low and variable blood flow, which further decreased irreversibly prior to the onset of pruning. Local blockade of blood flow with micro-bead obstruction led to vessel pruning, whereas increasing blood flow by noradrenergic elevation of heartbeat impeded the pruning process. Furthermore, the occurrence of vessel pruning could be largely predicted by haemodynamics-based numerical simulation of vasculature refinement. Thus, changes of blood flow drive vessel pruning via lateral migration of ECs, leading to the simplification of the vasculature and possibly efficient routing of blood flow in the developing brain.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.