ArticleJournal of bacteriology2012
Functional characterization of the stringent response regulatory gene dksA of Vibrio cholerae and its role in modulation of virulence phenotypes.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 50 citations in OpenAlex.
- DksA-Dependent Stringent Stress Response Drives Virulence and Gastrointestinal Persistence ofbioRxiv : the preprint server for biology · 2026Article
- (p)ppGpp and DksA play a crucial role in reducing the efficacy of β-lactam antibiotics by modulating bacterial membrane permeability.Microbiology spectrum · 2025Article
- Nucleoid-associated proteins shape the global protein occupancy and transcriptional landscape of a clinical isolate ofmSphere · 2024Article
- Fitness factors impacting survival of a subsurface bacterium in contaminated groundwater.The ISME journal · 2024Article
- Genomic and functional insights into antibiotic resistance genesMicrobiology (Reading, England) · 2024Article
- DksA, ppGpp, and RegAB Regulate Nitrate Respiration in Paracoccus denitrificans.Journal of bacteriology · 2023Article
- Similar and Divergent Roles of Stringent Regulator (p)ppGpp and DksA on Pleiotropic Phenotype of Yersinia enterocolitica.Microbiology spectrum · 2022Article
- The Pseudomonas aeruginosa DksA1 protein is involved in HScientific reports · 2022Article
- Expression and structure of the Chlamydia trachomatis DksA ortholog.Pathogens and disease · 2022Article
- DksA coordinates bile-mediated regulation of virulence-associated phenotypes in type three secretion system-positiveMicrobiology (Reading, England) · 2021Article
- Stringent response interacts with the ToxR regulon to regulate Vibrio cholerae virulence factor expression.Archives of microbiology · 2020Article
- Transcriptome analysis reveals that the RNA polymerase-binding protein DksA1 has pleiotropic functions inThe Journal of biological chemistry · 2020Article
- Functional Insights Into the Role ofFrontiers in microbiology · 2020Article
- (p)ppGpp Metabolism and Antimicrobial Resistance in Bacterial Pathogens.Frontiers in microbiology · 2020Review
- Stringent response regulators (p)ppGpp and DksA positively regulate virulence and host adaptation of Xanthomonas citri.Molecular plant pathology · 2019Article
- DksA Controls the Response of the Lyme Disease SpirocheteJournal of bacteriology · 2019Article
- QstR-dependent regulation of natural competence and type VI secretion in Vibrio cholerae.Nucleic acids research · 2018Article
- Genomic analysis of Acinetobacter baumannii prophages reveals remarkable diversity and suggests profound impact on bacterial virulence and fitness.Scientific reports · 2018Article
- Mechanisms of Bacterial Tolerance and Persistence in the Gastrointestinal and Respiratory Environments.Clinical microbiology reviews · 2018Review
- Guanosine tetra- and pentaphosphate increase antibiotic tolerance by reducing reactive oxygen species production inThe Journal of biological chemistry · 2018Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In bacteria, nutrient deprivation evokes the stringent response, which is mediated by the small intracellular signaling molecule ppGpp. In Gram negatives, the RelA enzyme synthesizes and SpoT hydrolyzes ppGpp, although the latter protein also has weak synthetase activity. DksA, a recently identified RNA polymerase binding transcription factor, acts as a coregulator along with ppGpp for controlling the stringent response. Recently, we have shown that three genes, relA, spoT, and relV, govern cellular levels of ppGpp during various starvation stresses in the Gram-negative cholera pathogen Vibrio cholerae. Here we report functional characterization of the dksA gene of V. cholerae (dksA(Vc)), coding for the protein DksA(Vc). Extensive genetic analyses of the ΔdksA(Vc) mutants suggest that DksA(Vc) is an important component involved in the stringent response in V. cholerae. Further analysis of mutants revealed that DksA(Vc) positively regulates various virulence-related processes, namely, motility, expression of the major secretory protease, called hemagglutinin protease (HAP), and production of cholera toxin (CT), under in vitro conditions. We found that DksA(Vc) upregulates expression of the sigma factor FliA (σ(28)), a critical regulator of motility in V. cholerae. Altogether, it appears that apart from stringent-response regulation, DksA(Vc) also has important roles in fine regulation of virulence-related phenotypes of V. cholerae.
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