ArticleJournal of bacteriology2012
Polynucleotide phosphorylase plays an important role in the generation of spontaneous mutations in Escherichia coli.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Polynucleotide Phosphorylase Stops Replication Restart by Degrading the RNA within R-loops during Replication-Transcription Conflicts.bioRxiv : the preprint server for biology · 2026Article
- Collective peroxide detoxification determines microbial mutation rate plasticity in E. coli.PLoS biology · 2024Article
- Strain Development in Microalgal Biotechnology-Random Mutagenesis Techniques.Life (Basel, Switzerland) · 2022Review
- Activity and Function in Human Cells of the Evolutionary Conserved Exonuclease Polynucleotide Phosphorylase.International journal of molecular sciences · 2022Review
- Specificity and mutagenesis bias of the mycobacterial alternative mismatch repair analyzed by mutation accumulation studies.Science advances · 2020Article
- Polynucleotide phosphorylase: Not merely an RNase but a pivotal post-transcriptional regulator.PLoS genetics · 2018Review
- Sources of spontaneous mutagenesis in bacteria.Critical reviews in biochemistry and molecular biology · 2018Review
- PNPase knockout results in mtDNA loss and an altered metabolic gene expression program.PloS one · 2018Article
- Polynucleotide phosphorylase is implicated in homologous recombination and DNA repair in Escherichia coli.BMC microbiology · 2017Article
- Anaerobically Grown Escherichia coli Has an Enhanced Mutation Rate and Distinct Mutational Spectra.PLoS genetics · 2017Article
- Mutagen Synergy: Hypermutability Generated by Specific Pairs of Base Analogs.Journal of bacteriology · 2016Article
- The small RNA SraG participates in PNPase homeostasis.RNA (New York, N.Y.) · 2016Article
- Mutational Consequences of Ciprofloxacin in Escherichia coli.Antimicrobial agents and chemotherapy · 2016Article
- Exploring Synergy between Classic Mutagens and Antibiotics To Examine Mechanisms of Synergy and Antibiotic Action.Antimicrobial agents and chemotherapy · 2015Article
- Deoxyribonucleotides as genetic and metabolic regulators.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Review
- Deoxycytidine deaminase-deficient Escherichia coli strains display acute sensitivity to cytidine, adenosine, and guanosine and increased sensitivity to a range of antibiotics, including vancomycin.Journal of bacteriology · 2014Article
- Distinctive effects of domain deletions on the manganese-dependent DNA polymerase and DNA phosphorylase activities of Mycobacterium smegmatis polynucleotide phosphorylase.Biochemistry · 2013Article
- Rate and molecular spectrum of spontaneous mutations in the bacterium Escherichia coli as determined by whole-genome sequencing.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Extreme dNTP pool changes and hypermutability in dcd ndk strains.Mutation researchArticle
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polynucleotide phosphorylase (PNP) plays a central role in RNA degradation, generating a pool of ribonucleoside diphosphates (rNDPs) that can be converted to deoxyribonucleoside diphosphates (dNDPs) by ribonucleotide reductase. We report here that spontaneous mutations resulting from replication errors, which are normally repaired by the mismatch repair (MMR) system, are sharply reduced in a PNP-deficient Escherichia coli strain. This is true for base substitution mutations that occur in the rpoB gene leading to Rif(r) and the gyrB gene leading to Nal(r) and for base substitution and frameshift mutations that occur in the lacZ gene. These results suggest that the increase in the rNDP pools generated by polynucleotide phosphorylase (PNP) degradation of RNA is responsible for the spontaneous mutations observed in an MMR-deficient background. The PNP-derived pool also appears responsible for the observed mutations in the mutT mutator background and those that occur after treatment with 5-bromodeoxyuridine, as these mutations are also drastically reduced in a PNP-deficient strain. However, mutation frequencies are not reduced in a mutY mutator background or after treatment with 2-aminopurine. These results highlight the central role in mutagenesis played by the rNDP pools (and the subsequent dNTP pools) derived from RNA degradation.
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