ArticleNucleic acids research2012
Genome-wide identification of genes directly regulated by the pleiotropic transcription factor Spx in Bacillus subtilis.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed, 101 citations in OpenAlex.
- Stress-induced ribosome degradation in Bacillus subtilis is mediated by the RNase Y-specificity complex.Nature communications · 2026Article
- Spx inhibits expression of the SwrA•DegU master flagellar activator inJournal of bacteriology · 2026Article
- Activation of the Spx redox sensor counters cysteine-driven Fe(II) depletion under disulfide stress.bioRxiv : the preprint server for biology · 2025Article
- Reductive activation of the disulfide-containing antibiotic thiolutin is mediated by both bacillithiol and FAD-dependent disulfide reductases.Journal of bacteriology · 2025Article
- Characterization of the MgsR-dependent promoter structure in Bacillus subtilis-application of a novel pHIS plasmid-based screening system for promoter element analysis.Nucleic acids research · 2025Article
- The CssRS two-component system of Bacillus subtilis contributes to teicoplanin and polymyxin B response.Folia microbiologica · 2025Article
- Requirement of ClpX for CtsR dissociation from its operator elements upon heat stress inFrontiers in microbiology · 2025Article
- LiaR-dependent gene expression contributes to antimicrobial responses in group AAntimicrobial agents and chemotherapy · 2024Article
- Article
- Greedy reduction of Bacillus subtilis genome yields emergent phenotypes of high resistance to a DNA damaging agent and low evolvability.Nucleic acids research · 2023Article
- (p)ppGpp - an important player during heat shock response.microLife · 2023Review
- Nanobiotics against antimicrobial resistance: harnessing the power of nanoscale materials and technologies.Journal of nanobiotechnology · 2022Review
- Sulfur Availability Impacts Accumulation of the 2-Thiouridine tRNA Modification in Bacillus subtilis.Journal of bacteriology · 2022Article
- Update on the Protein Homeostasis Network inFrontiers in microbiology · 2022Review
- Structural basis of transcription activation by the global regulator Spx.Nucleic acids research · 2021Article
- The Division Defect of aJournal of bacteriology · 2021Article
- Statistical modelling of bacterial promoter sequences for regulatory motif discovery with the help of transcriptome data: application toJournal of the Royal Society, Interface · 2020Article
- Listeria monocytogenes SpxA1 is a global regulator required to activate genes encoding catalase and heme biosynthesis enzymes for aerobic growth.Molecular microbiology · 2020Article
- Regulatory circuits controlling Spx levels in Streptococcus mutans.Molecular microbiology · 2020Article
- YjbH Requires Its Thioredoxin Active Motif for the Nitrosative Stress Response, Cell-to-Cell Spread, and Protein-Protein Interactions in Listeria monocytogenes.Journal of bacteriology · 2020Article
4 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transcriptional regulator Spx plays a key role in maintaining the redox homeostasis of Bacillus subtilis cells exposed to disulfide stress. Defects in Spx were previously shown to lead to differential expression of numerous genes but direct and indirect regulatory effects could not be distinguished. Here we identified 283 discrete chromosomal sites potentially bound by the Spx-RNA polymerase (Spx-RNAP) complex using chromatin immunoprecipitation of Spx. Three quarters of these sites were located near Sigma(A)-dependent promoters, and upon diamide treatment, the fraction of the Spx-RNAP complex increased in parallel with the number and occupancy of DNA sites. Correlation of Spx-RNAP-binding sites with gene differential expression in wild-type and Δspx strains exposed or not to diamide revealed that 144 transcription units comprising 275 genes were potentially under direct Spx regulation. Spx-controlled promoters exhibited an extended -35 box in which nucleotide composition at the -43/-44 positions strongly correlated with observed activation. In vitro transcription confirmed activation by oxidized Spx of seven newly identified promoters, of which one was also activated by reduced Spx. Our study globally characterized the Spx regulatory network, revealing its role in the basal expression of some genes and its complex interplay with other stress responses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.