ArticleNucleic acids research2012
Helicobacter pylori oriC--the first bipartite origin of chromosome replication in Gram-negative bacteria.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Robust helicase loading mechanism underlies diverse architectures of bacterial replication origins.Nucleic acids research · 2026Article
- Structures of DnaA domain I reveal a dimer conserved across Actinomycetes.Nucleic acids research · 2026Article
- Profiling of the Helicobacter pylori redox switch HP1021 regulon using a multi-omics approach.Nature communications · 2023Article
- HU Knew? Bacillus subtilis HBsu Is Required for DNA Replication Initiation.Journal of bacteriology · 2022Article
- Putative Cooperative ATP-DnaA Binding to Double-Stranded DnaA Box and Single-Stranded DnaA-Trio Motif uponInternational journal of molecular sciences · 2021Article
- Transcriptional Activity of the Bacterial Replication Initiator DnaA.Frontiers in microbiology · 2021Review
- gammaBOriS: Identification and Taxonomic Classification of Origins of Replication in Gammaproteobacteria using Motif-based Machine Learning.Scientific reports · 2020Article
- Identification of the Unwinding Region in theFrontiers in microbiology · 2020Article
- Identification of a basal system for unwinding a bacterial chromosome origin.The EMBO journal · 2019Article
- The core genome m5C methyltransferase JHP1050 (M.Hpy99III) plays an important role in orchestrating gene expression in Helicobacter pylori.Nucleic acids research · 2019Article
- Changing Perspectives on the Role of DnaA-ATP in Orisome Function and Timing Regulation.Frontiers in microbiology · 2019Review
- The Origin of Chromosomal Replication Is Asymmetrically Positioned on the Mycobacterial Nucleoid, and the Timing of Its Firing Depends on HupB.Journal of bacteriology · 2018Article
- Where and When Bacterial Chromosome Replication Starts: A Single Cell Perspective.Frontiers in microbiology · 2018Review
- Structure and Function of theFrontiers in microbiology · 2018Article
- Non-Random Inversion Landscapes in Prokaryotic Genomes Are Shaped by Heterogeneous Selection Pressures.Molecular biology and evolution · 2017Article
- Control of bacterial chromosome replication by non-coding regions outside the origin.Current genetics · 2017Review
- Review
- Review
- The bacterial DnaA-trio replication origin element specifies single-stranded DNA initiator binding.Nature · 2016Article
- 'Modulation of the enzymatic activities of replicative helicase (DnaB) by interaction with Hp0897: a possible mechanism for helicase loading in Helicobacter pylori'.Nucleic acids research · 2016Article
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Binding of the DnaA protein to oriC leads to DNA melting within the DNA unwinding element (DUE) and initiates replication of the bacterial chromosome. Helicobacter pylori oriC was previously identified as a region localized upstream of dnaA and containing a cluster of DnaA boxes bound by DnaA protein with a high affinity. However, no unwinding within the oriC sequence has been detected. Comprehensive in silico analysis presented in this work allowed us to identify an additional region (oriC2), separated from the original one (oriC1) by the dnaA gene. DnaA specifically binds both regions, but DnaA-dependent DNA unwinding occurs only within oriC2. Surprisingly, oriC2 is bound exclusively as supercoiled DNA, which directly shows the importance of the DNA topology in DnaA-oriC interactions, similarly as previously presented only for initiator-origin interactions in Archaea and some Eukaryota. We conclude that H. pylori oriC exhibits bipartite structure, being the first such origin discovered in a Gram-negative bacterium. The H. pylori mode of initiator-oriC interactions, with the loop formation between the subcomplexes of the discontinuous origin, resembles those discovered in Bacillus subtilis chromosome and in many plasmids, which might suggest a similar way of controlling initiation of replication.
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