ArticleNucleic acids research2012
The slicing activity of miRNA-specific Argonautes is essential for the miRNA pathway in C. elegans.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 46 citations in OpenAlex.
- Dynamic control of Argonautes by a rapidly evolving immunological switch.Current biology : CB · 2025Article
- A guide to the biogenesis and functions of endogenous small non-coding RNAs in animals.Nature reviews. Molecular cell biology · 2025Review
- Catalytic residues of microRNA Argonautes play a modest role in microRNA star strand destabilization in C. elegans.Nucleic acids research · 2024Article
- Defining the contribution of microRNA-specific Argonautes with slicer capability in animals.Nucleic acids research · 2024Article
- Modeling neurodevelopmental disorder-associated humanProceedings of the National Academy of Sciences of the United States of America · 2024Article
- The catalytic activity of microRNA Argonautes plays a modest role in microRNA star strand destabilization inbioRxiv : the preprint server for biology · 2024Article
- Systematic characterization of small RNAs associated with C. elegans Argonautes.Science China. Life sciences · 2023Article
- MicroRNA biogenesis pathway alterations in aging.Extracellular vesicles and circulating nucleic acids · 2023Review
- A specific type of Argonaute phosphorylation regulates binding to microRNAs during C. elegans development.Cell reports · 2022Article
- A Structural View of miRNA Biogenesis and Function.Non-coding RNA · 2022Review
- Germline inherited small RNAs facilitate the clearance of untranslated maternal mRNAs in C. elegans embryos.Nature communications · 2021Article
- The Argonaute Proteins ALG-1 and ALG-2 Are Linked to Stress Resistance and Proteostasis.microPublication biology · 2021Article
- Mechanistic Insights into MicroRNA-Mediated Gene Silencing.Cold Spring Harbor perspectives in biology · 2019Review
- The Nematode Caenorhabditis elegans as a Model Organism to Study Metabolic Effects of ω-3 Polyunsaturated Fatty Acids in Obesity.Advances in nutrition (Bethesda, Md.) · 2019Review
- ALG-5 is a miRNA-associated Argonaute required for proper developmental timing in the Caenorhabditis elegans germline.Nucleic acids research · 2017Article
- Exploring the mitochondrial microRNA import pathway through Polynucleotide Phosphorylase (PNPase).Journal of molecular and cellular cardiology · 2017Article
- Phosphorylation of Argonaute proteins affects mRNA binding and is essential for microRNA-guided gene silencingThe EMBO journal · 2017Article
- The microRNA machinery regulates fasting-induced changes in gene expression and longevity inThe Journal of biological chemistry · 2017Article
- Neuronal function of the mRNA decapping complex determines survival ofOpen biology · 2017Article
- TEG-1 CD2BP2 controls miRNA levels by regulating miRISC stability in C. elegans and human cells.Nucleic acids research · 2017Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Among the set of Argonautes proteins encoded by metazoan genomes, some have conserved amino acids important for catalytic or slicing activity. The functional significance of these residues in microRNA (miRNA)-specific Argonautes in animals is still unclear since miRNAs do not induce site-specific cleavage of targeted messenger RNAs (mRNAs), unlike small interfering RNAs (siRNAs). Here, we report that miRNA-specific ALG-1 and ALG-2 Argonautes from Caenorhabditis elegans possess the slicing activity normally implicated in the siRNA-silencing pathway. We also find that ALG-1/2 can bind and use a Dicer-processed miRNA duplex to target mRNAs, suggesting an ability to displace RNA strands. Importantly, the slicing activity of ALG-1 or ALG-2 is essential for the miRNA pathway in vivo, as shown by the accumulation of truncated miRNA precursors and altered miRNA-induced silencing complex (miRISC) formation. Taken together, our data demonstrate that the slicing activity of Argonautes contributes to a new and unexpected step in the canonical miRNA pathway that occurs prior to miRISC loading in animals.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.