ArticleGenetics2012
High-throughput discovery of mutations in tef semi-dwarfing genes by next-generation sequencing analysis.
Article in Genetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 60 citations in OpenAlex.
- Elevated accumulation of lutein and zeaxanthin in a novel high-biomass yielding strain Dunaliella sp. ZP-1 obtained through EMS mutagenesis.Biotechnology for biofuels and bioproducts · 2025Article
- Article
- CRISPR/Cas9-mediated tetra-allelic mutation of the 'Green Revolution' SEMIDWARF-1 (SD-1) gene confers lodging resistance in tef (Eragrostis tef).Plant biotechnology journal · 2022Article
- CRISPR for accelerating genetic gains in under-utilized crops of the drylands: Progress and prospects.Frontiers in genetics · 2022Review
- From Traditional Breeding to Genome Editing for Boosting Productivity of the Ancient Grain Tef [Plants (Basel, Switzerland) · 2021Article
- Genome sequence and genetic diversity analysis of an under-domesticated orphan crop, white fonio (Digitaria exilis).GigaScience · 2021Article
- Application of Genome Editing in Tomato Breeding: Mechanisms, Advances, and Prospects.International journal of molecular sciences · 2021Review
- Exceptional subgenome stability and functional divergence in the allotetraploid Ethiopian cereal teff.Nature communications · 2020Article
- Multiplex exome sequencing reveals genome-wide frequency and distribution of mutations in the 'Micro-Tom' Targeting Induced Local Lesions in Genomes (TILLING) mutant library.Plant biotechnology (Tokyo, Japan) · 2019Article
- Significance and prospects of an orphan crop tef.Planta · 2019Review
- Improving Lodging Resistance: Using Wheat and Rice as Classical Examples.International journal of molecular sciences · 2019Review
- Structural Characterization ofG3 (Bethesda, Md.) · 2019Article
- Forward Genetics by Sequencing EMS Variation-Induced Inbred Lines.G3 (Bethesda, Md.) · 2017Article
- Exploring Genetic Diversity in Plants Using High-Throughput Sequencing Techniques.Current genomics · 2016Article
- Identification and characterization of abundant repetitive sequences in Eragrostis tef cv. Enatite genome.BMC plant biology · 2016Article
- Construction of a high-density mutant library in soybean and development of a mutant retrieval method using amplicon sequencing.BMC genomics · 2015Article
- Ion Torrent sequencing as a tool for mutation discovery in the flax (Linum usitatissimum L.) genome.Plant methods · 2015Article
- Proteomic analysis of middle and late stages of bread wheat (Triticum aestivum L.) grain development.Frontiers in plant science · 2015Article
- Forward genetics by genome sequencing reveals that rapid cyanide release deters insect herbivory of Sorghum bicolor.Genetics · 2013Article
- A mutant Brassica napus (canola) population for the identification of new genetic diversity via TILLING and next generation sequencing.PloS one · 2013Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tef (Eragrostis tef) is a major cereal crop in Ethiopia. Lodging is the primary constraint to increasing productivity in this allotetraploid species, accounting for losses of ∼15-45% in yield each year. As a first step toward identifying semi-dwarf varieties that might have improved lodging resistance, an ∼6× fosmid library was constructed and used to identify both homeologues of the dw3 semi-dwarfing gene of Sorghum bicolor. An EMS mutagenized population, consisting of ∼21,210 tef plants, was planted and leaf materials were collected into 23 superpools. Two dwarfing candidate genes, homeologues of dw3 of sorghum and rht1 of wheat, were sequenced directly from each superpool with 454 technology, and 120 candidate mutations were identified. Out of 10 candidates tested, six independent mutations were validated by Sanger sequencing, including two predicted detrimental mutations in both dw3 homeologues with a potential to improve lodging resistance in tef through further breeding. This study demonstrates that high-throughput sequencing can identify potentially valuable mutations in under-studied plant species like tef and has provided mutant lines that can now be combined and tested in breeding programs for improved lodging resistance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.