ArticleEukaryotic cell2012
Structural requirements for the activity of the MirB ferrisiderophore transporter of Aspergillus fumigatus.
Article in Eukaryotic cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 62 citations in OpenAlex.
- Review
- Fission yeast cells deficient in siderophore biosynthesis require Str2 for ferrichrome-dependent growth.Frontiers in microbiology · 2025Article
- Siderophore Biosynthesis and Transport Systems in Model and Pathogenic Fungi.Journal of microbiology and biotechnology · 2024Review
- Regulatory and pathogenic mechanisms in response to iron deficiency and excess in fungi.Microbial biotechnology · 2023Review
- Article
- The Siderophore Ferricrocin Mediates Iron Acquisition in Aspergillus fumigatus.Microbiology spectrum · 2023Article
- Molecular Regulatory Mechanism of the Iron-Ion-Promoted Asexual Sporulation ofJournal of fungi (Basel, Switzerland) · 2023Article
- Article
- GNPS-Guided Discovery of Madurastatin Siderophores from the Termite-Associated Actinomadura sp. RB99.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Article
- Antibacterial diphenyl ether production induced by co-culture of Aspergillus nidulans and Aspergillus fumigatus.Applied microbiology and biotechnology · 2022Article
- The Siderophore Transporters Sit1 and Sit2 Are Essential for Utilization of Ferrichrome-, Ferrioxamine- and Coprogen-Type Siderophores inJournal of fungi (Basel, Switzerland) · 2021Article
- Antifungal Siderophore Conjugates for Theranostic Applications in Invasive Pulmonary Aspergillosis Using Low-Molecular TAFC Scaffolds.Journal of fungi (Basel, Switzerland) · 2021Article
- Antifungal Activity of Siderophore Isolated FromFrontiers in microbiology · 2021Article
- Siderophore Scaffold as Carrier for Antifungal Peptides in Therapy ofJournal of fungi (Basel, Switzerland) · 2020Article
- Combinatorial Biosynthesis of Sulfated Benzenediol Lactones with a Phenolic Sulfotransferase from Fusarium graminearum PH-1.mSphere · 2020Article
- The "Little Iron Waltz": The Ternary Response ofJournal of fungi (Basel, Switzerland) · 2020Review
- Siderophore-Based Molecular Imaging of Fungal and Bacterial Infections-Current Status and Future Perspectives.Journal of fungi (Basel, Switzerland) · 2020Review
- Hybrid Imaging ofBiomolecules · 2020Article
- Modifying the Siderophore Triacetylfusarinine C for Molecular Imaging of Fungal Infection.Molecular imaging and biology · 2019Article
- The Siderophore Transporter Sit1 Determines Susceptibility to the Antifungal VL-2397.Antimicrobial agents and chemotherapy · 2019Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Siderophores have been identified as virulence factors in the opportunistic fungal pathogen Aspergillus fumigatus. The 14-pass transmembrane protein MirB is postulated to function as a siderophore transporter, responsible for uptake of the hydroxamate siderophore N,N',N″-triacetylfusarinine C (TAFC). Our aim was to identify amino acids of A. fumigatus MirB that are crucial for uptake of TAFC. Site-directed mutagenesis was used to create MirB mutants. Expression of wild-type and mutant proteins in the Saccharomyces cerevisiae strain PHY14, which lacks endogenous siderophore transporters, was confirmed by Western blotting. TAFC transport assays using (55)Fe-labeled TAFC and growth assays with Fe-TAFC as the sole iron source identified alanine 125, tyrosine 577, loop 3, and the second half of loop 7 (Loop7Del2) as crucial for function, since their substitution or deletion abrogated uptake completely. Wild-type MirB transported ferricrocin and coprogen as well as TAFC but not ferrichrysin. MirB was localized by fluorescence microscopy using antisera raised against a MirB extracellular loop peptide. Immunofluorescence microscopy showed that in yeast, wild-type MirB had a punctate distribution under the plasma membrane, as did the A125D and Y577A strains, indicating that the defect in transport of these mutants was unlikely to be due to mislocalization or degradation. MirB immunolocalization in A. fumigatus showed that the transporter was found in vesicles which cycled between the cytoplasm and the plasma membrane and was concentrated at the hyphal tips. The location of MirB was not influenced by the presence of the siderophore TAFC but was sensitive to internal iron stores.
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