ArticleParasitology research2012
Sequencing for complete rDNA sequences (18S, ITS1, 5.8S, ITS2, and 28S rDNA) of Demodex and phylogenetic analysis of Acari based on 18S and 28S rDNA.
Article in Parasitology research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 34 citations in OpenAlex.
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- The nuclear 28S gene fragment D3 as species marker in oribatid mites (Acari, Oribatida) from German peatlands.Experimental & applied acarology · 2017Article
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- Molecular identification and phylogenetic study of Demodex caprae.Parasitology research · 2014Article
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- Utility of divergent domains of 28S ribosomal RNA in species discrimination of paramphistomes (Trematoda: Digenea: Paramphistomoidea).Parasitology research · 2013Article
- Phylogenetic analysis of Demodex caprae based on mitochondrial 16S rDNA sequence.Parasitology research · 2013Article
- Molecular identification of four phenotypes of human Demodex mites (Acari: Demodicidae) based on mitochondrial 16S rDNA.Parasitology research · 2013Article
- Discrimination between Demodex folliculorum (Acari: Demodicidae) isolates from China and Spain based on mitochondrial cox1 sequences.Journal of Zhejiang University. Science. B · 2013Article
- The first case of Demodex gatoi in Austria, detected with fecal flotation.Parasitology research · 2013Article
- Characterization of active ribosomal RNA harboring MITEs insertion in microsporidian Nosema bombycis genome.Parasitology research · 2013Article
- Morphologic and Molecular Characterization of a Demodex (Acari: Demodicidae) Species from White-Tailed Deer (Odocoileus virginianus).ISRN parasitology · 2013Article
- Cloning and sequence analysis of chitin synthase gene fragments of Demodex mites.Journal of Zhejiang University. Science. B · 2012Article
- Molecular identification and phylogenetic relationship ofTropical parasitologyArticle
- Molecular Characterization and Analysis of 16S Ribosomal DNA in Some Isolates ofIranian journal of parasitologyArticle
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Due to the difficulty of DNA extraction for Demodex, few studies dealt with the identification and the phyletic evolution of Demodex at molecular level. In this study, we amplified, sequenced, and analyzed a complete (Demodex folliculorum) and an almost complete (D12 missing) (Demodex brevis) ribosomal DNA (rDNA) sequence and also analyzed the primary sequences of divergent domains in small-subunit ribosomal RNA (rRNA) of 51 species and in large-subunit rRNA of 43 species from four superfamilies in Acari (Cheyletoidea, Tetranychoidea, Analgoidea, and Ixodoidea). The results revealed that 18S rDNA sequence was relatively conserved in rDNA-coding regions and was not evolving as rapidly as 28S rDNA sequence. The evolutionary rates of transcribed spacer regions were much higher than those of the coding regions. The maximum parsimony trees of 18S and 28S rDNA appeared to be almost identical, consistent with their morphological classification. Based on the fact that the resolution capability of sequence length and the divergence of the 13 segments (D1-D6, D7a, D7b, and D8-D12) of 28S rDNA were stronger than that of the nine variable regions (V1-V9) of 18S rDNA, we were able to identify Demodex (Cheyletoidea) by the indels occurring in D2, D6, and D8.
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