ArticleNeuromolecular medicine2013
RVG-mediated calpain2 gene silencing in the brain impairs learning and memory.
Article in Neuromolecular medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 26 citations in OpenAlex.
- Extracellular vesicle-based delivery of silencing sequences for the treatment of Machado-Joseph disease/spinocerebellar ataxia type 3.Molecular therapy : the journal of the American Society of Gene Therapy · 2023Article
- Prenatal Hypoxia and Placental Oxidative Stress: Insights from Animal Models to Clinical Evidences.Antioxidants (Basel, Switzerland) · 2020Review
- Identification of a Peptide for Systemic Brain Delivery of a Morpholino Oligonucleotide in Mouse Models of Spinal Muscular Atrophy.Nucleic acid therapeutics · 2017Article
- Differential role of calpain-dependent protein cleavage in intermediate and long-term operant memory in Aplysia.Neurobiology of learning and memory · 2017Article
- Article
- Erythropoietin Modulates Cerebral and Serum Degradation Products from Excess Calpain Activation following Prenatal Hypoxia-Ischemia.Developmental neuroscience · 2016Article
- A critical evaluation of drug delivery from ligand modified nanoparticles: Confounding small molecule distribution and efficacy in the central nervous system.Journal of controlled release : official journal of the Controlled Release Society · 2015Article
- K-Cl cotransporter KCC2--a moonlighting protein in excitatory and inhibitory synapse development and function.Pflugers Archiv : European journal of physiology · 2015Review
- A method for concentrating lipid peptide DNA and siRNA nanocomplexes that retains their structure and transfection efficiency.International journal of nanomedicine · 2015Article
- Learning and memory: an emergent property of cell motility.Neurobiology of learning and memory · 2013Review
- Targeting calpain in synaptic plasticity.Expert opinion on therapeutic targets · 2013Review
- Conditional disruption of calpain in the CNS alters dendrite morphology, impairs LTP, and promotes neuronal survival following injury.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Calpain-2-mediated PTEN degradation contributes to BDNF-induced stimulation of dendritic protein synthesis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
In the central nervous system, two calpain isoforms are highly expressed: calpain1 and calpain2. Here, we show for the first time that activation of the calpain isoform, calpain2, is a necessary event in hippocampal synaptic plasticity and in learning and memory. We developed a fluorescence resonance energy transfer-based animal model to monitor in vivo calpain activation in single cells and in real time. Additionally, utilizing a novel rabies virus glycoprotein-chimeric peptide, which enabled the transvascular delivery of small interfering RNA to the brain against calpain2, we down-regulated the calpain2 isoform in vivo. Calpain2 gene silencing eliminated long-term potentiation and impaired learning and memory. Our results not only identify the calpain2 isoform as a critical mediator in learning and memory but also highlight an innovative, highly efficient calpain2-targeting peptide capable of isoform-specific gene silencing in the brain. We anticipate these innovative technologies and our better understanding of the calpain machinery, particularly of the calpain2 isoform, will have substantial influence on future translational studies, attracting considerable interest in the use of calpain models and calpain-specific inhibitors in the development of therapeutics.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.