ArticleDigestive diseases and sciences2013
Zinc supplementation modifies tight junctions and alters barrier function of CACO-2 human intestinal epithelial layers.
Article in Digestive diseases and sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
49 citing papers in PubMed, 1 synthesis or guideline pooled it, 116 citations in OpenAlex.
- Pooled it
- Food-gut continuum of biofortified micronutrients: Influence of breadmaking processes on iodine, selenium, and zinc bioaccessibility and epithelial responses in anFood chemistry: X · 2026Article
- Rectal delivered nanocomposite suppository against radiation-induced proctitis.Journal of nanobiotechnology · 2026Article
- Orally Administered Zinc Gluconate Induces Tight Junctional Remodeling and Reduces Passive Transmucosal Permeability Across Human Intestine in a Patient-Based Study.International journal of molecular sciences · 2025Article
- Impact of a New Preoperative Immune-Nutrition Protocol Using Zinc on Hospital Outcomes of Children with Hirschsprung's Disease: A Novel Randomized Controlled Trial.Pediatric gastroenterology, hepatology & nutrition · 2025Article
- Immunomodulatory Effects of Vitamin D and Zinc on Viral Infection.Biological trace element research · 2025Review
- Micronutrients at Supplemental Levels, Tight Junctions and Epithelial Barrier Function: A Narrative Review.International journal of molecular sciences · 2024Review
- Potential Complementary Effect of Zinc andNutrients · 2024Review
- Effects of wheat germ diet on intestinal antioxidant capacity, immunological function and gut microbiota of Sichuan white geese.Frontiers in microbiology · 2024Article
- Protective effect of zinc gluconate on intestinal mucosal barrier injury in antibiotics and LPS-induced mice.Frontiers in microbiology · 2024Article
- Aryl hydrocarbon receptor utilises cellular zinc signals to maintain the gut epithelial barrier.Nature communications · 2023Article
- ZnUMBA - a live imaging method to detect local barrier breaches.Journal of cell science · 2023Article
- Enterocyte-specific deletion of metal transporter Zip14 (Slc39a14) alters intestinal homeostasis through epigenetic mechanisms.American journal of physiology. Gastrointestinal and liver physiology · 2023Article
- TEER and Ion Selective Transwell-Integrated Sensors System for Caco-2 Cell Model.Micromachines · 2023Article
- Early supplementation with zinc proteinate does not change rectal microbiota but increases growth performance by improving antioxidant capacity and plasma zinc concentration in preweaned dairy calves.Frontiers in veterinary science · 2023Article
- Stress mechanism involved in the progression of alcoholic liver disease and the therapeutic efficacy of nanoparticles.Frontiers in immunology · 2023Review
- Dietary supplementation of ferrous glycinate improves intestinal barrier function by modulating microbiota composition in Cherry Valley ducks.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2022Article
- Micronutrient Improvement of Epithelial Barrier Function in Various Disease States: A Case for Adjuvant Therapy.International journal of molecular sciences · 2022Review
- Multiple Micronutrient Supplementation: As a Supportive Therapy in the Treatment of COVID-19.BioMed research international · 2022Review
- Comparative effect of potentiated zinc oxide and antibiotic growth promoters on intestinal morphometry and nutrient digestibility in broiler chickens.Tropical animal health and production · 2021Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundZinc deficiency is known to result in epithelial barrier leak in the GI tract. Precise effects of zinc on epithelial tight junctions (TJs) are only beginning to be described and understood. Along with nutritional regimens like methionine-restriction and compounds such as berberine, quercetin, indole, glutamine and rapamycin, zinc has the potential to function as a TJ modifier and selective enhancer of epithelial barrier function.
aimsThe purpose of this study was to determine the effects of zinc-supplementation on the TJs of a well-studied in vitro GI model, CACO-2 cells.
methodsBarrier function was assessed electrophysiologically by measuring transepithelial electrical resistance (Rt), and radiochemically, by measuring transepithelial (paracellular) diffusion of 14C-D-mannitol and 14C-polyethyleneglycol. TJ composition was studied by Western immunoblot analyses of occludin, tricellulin and claudins-1 to -5 and -7.
resultsFifty- and 100-μM zinc concentrations (control medium is 2 μM) significantly increase Rt but simultaneously increase paracellular leak to D-mannitol. Claudins 2 and 7 are downregulated in total cell lysates, while occludin, tricellulin and claudins-1, -3, -4 and -5 are unchanged. Claudins-2 and -7 as well as tricellulin exhibit decreased cytosolic content as a result of zinc supplementation.
conclusionsZinc alters CACO-2 TJ composition and modifies TJ barrier function selectively. Zinc is one of a growing number of "nutraceutical" substances capable of enhancing epithelial barrier function, and may find use in countering TJ leakiness induced in various disease states.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.