ArticleNature medicine2012
On silico peptide microarrays for high-resolution mapping of antibody epitopes and diverse protein-protein interactions.
Article in Nature medicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
55 citing papers in PubMed, 103 citations in OpenAlex.
- Analytical validation of a multiplexed peptide microarray for multi-antigen Epstein-Barr virus serological profiling across diverse clinical specimens.Frontiers in immunology · 2026Article
- Acid-Modulated Peptide Synthesis for Application on Oxide Biosensor Interfaces.Nanomaterials (Basel, Switzerland) · 2023Article
- Miniaturized Control of Acidity in Multiplexed Microreactors.ACS omega · 2023Article
- Applications of Peptide Microarrays in Autoantibody, Infection, and Cancer Detection.Methods in molecular biology (Clifton, N.J.) · 2023Review
- High Interferon Signature Leads to Increased STAT1/3/5 Phosphorylation in PBMCs From SLE Patients by Single Cell Mass Cytometry.Frontiers in immunology · 2022Article
- Extracellular histones aggravate autoimmune arthritis by lytic cell death.Frontiers in immunology · 2022Article
- High-throughput determination of protein affinities using unmodified peptide libraries in nanomolar scale.iScience · 2021Article
- Epitope-resolved profiling of the SARS-CoV-2 antibody response identifies cross-reactivity with endemic human coronaviruses.Cell reports. Medicine · 2021Article
- Exploring the Ligand Preferences of the PHD1 Domain of Histone Demethylase KDM5A Reveals Tolerance for Modifications of the Q5 Residue of Histone 3.ACS chemical biology · 2021Article
- Applications of Protein Microarrays in Biomarker Discovery for Autoimmune Diseases.Frontiers in immunology · 2021Review
- Models to inform neutralizing antibody therapy strategies during pandemics: the case of SARS-CoV-2.Antibody therapeutics · 2021Article
- Rapid Response to Pandemic Threats: Immunogenic Epitope Detection of Pandemic Pathogens for Diagnostics and Vaccine Development Using Peptide Microarrays.Journal of proteome research · 2020Review
- Immunological fingerprint of 4CMenB recombinant antigens via protein microarray reveals key immunosignatures correlating with bactericidal activity.Nature communications · 2020Article
- Implications of antibody-dependent enhancement of infection for SARS-CoV-2 countermeasures.Nature biotechnology · 2020Article
- Quantitative mapping of protein-peptide affinity landscapes using spectrally encoded beads.eLife · 2019Article
- Detection of epitopes in systemic lupus erythematosus using peptide microarray.Molecular medicine reports · 2018Article
- Experimental characterization and simulation of amino acid and peptide interactions with inorganic materials.Engineering in life sciences · 2018Review
- Electric fields control the orientation of peptides irreversibly immobilized on radical-functionalized surfaces.Nature communications · 2018Article
- Peptide Arrays: Development and Application.Analytical chemistry · 2018Review
- A Simple Platform for the Rapid Development of Antimicrobials.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
We developed a new, silicon-based peptide array for a broad range of biological applications, including potential development as a real-time point-of-care platform. We used photolithography on silicon wafers to synthesize microarrays (Intel arrays) that contained every possible overlapping peptide within a linear protein sequence covering the N-terminal tail of human histone H2B. These arrays also included peptides with acetylated and methylated lysine residues, reflecting post-translational modifications of H2B. We defined minimum binding epitopes for commercial antibodies recognizing the modified and unmodified H2B peptides. We further found that this platform is suitable for the highly sensitive characterization of methyltransferases and kinase substrates. The Intel arrays also revealed specific H2B epitopes that are recognized by autoantibodies in individuals with systemic lupus erythematosus who have elevated disease severity. By combining emerging nonfluorescence-based detection methods with an underlying integrated circuit, we are now poised to create a truly transformative proteomics platform with applications in bioscience, drug development and clinical diagnostics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.