ArticleActa biomaterialia2013
Tuning three-dimensional collagen matrix stiffness independently of collagen concentration modulates endothelial cell behavior.
Article in Acta biomaterialia, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 179 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
179 citing papers in PubMed.
- Matrix degradation promotes fibronectin deposition and spatial remodeling in 3D.Cell reports. Physical science · 2026Article
- Angiogenic Potential of Endothelial Cells in Response to the Stiffness and Anisotropy of Right Ventricle Mimetic Scaffolds.Bioengineering (Basel, Switzerland) · 2026Article
- Heterogeneous evolution of pancreatic cancer microvasculature revealed by longitudinal intravital imaging.Cancer cell international · 2026Article
- Advances in GelMA Hydrogel-Enabled Angiogenic-Osteogenic Coupling: From Structural Programming to Exogenous Cue Synergy.Journal of functional biomaterials · 2026Review
- Genipin-Crosslinked, Silane-Anchored 3D Tumor-Stroma Microtissues for High-Content On-Chip Drug Testing.Advanced healthcare materials · 2026Article
- An Advanced 3D Model of Vascularized Epithelial Ovarian Cancer in a Tumor-on-a-Chip System Based on Multi-Cell Culture.Sensors (Basel, Switzerland) · 2026Article
- Generation of In Vivo-Inspired 3D Collagen Models for Guided Tumor Invasion In Vitro.Current protocols · 2026Article
- Diabetic hyperglycemia-induced glycation regulates tumor vasculature integrity via NF-κB-mediated GM-CSF secretion by tumor cells.Cell biomaterials · 2025Article
- Characterizing the Impact of Fabrication Methods on Mechanically Tunable Gelatin Hydrogels for Cardiac Fibrosis Studies.Bioengineering (Basel, Switzerland) · 2025Article
- Matrix degradation enhances stress relaxation, regulating cell adhesion and spreading.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- An algorithmic and software framework to incorporate orientation distribution functions in finite element simulations for biomechanics and biophysics.Acta biomaterialia · 2025Article
- Microvascular endothelial cells display organ-specific responses to extracellular matrix stiffness.Current research in physiology · 2025Article
- Human Retinal Progenitor Cell (hRPC) Migration in Three-Dimensional (3D) Environments of Varying Stiffness and Composition.Journal of tissue engineering and regenerative medicine · 2025Article
- A hybrid computational model of cancer spheroid growth with ribose-induced collagen stiffening.Frontiers in bioengineering and biotechnology · 2025Article
- YAP/TAZ drives Notch and angiogenesis mechanoregulation in silico.NPJ systems biology and applications · 2024Article
- Complement factor H in molecular regulation of angiogenesis.Medical review (2021) · 2024Review
- Standardizing designed and emergent quantitative features in microphysiological systems.Nature biomedical engineering · 2024Review
- Efficient Generation of Skin Organoids from Pluripotent Cells via Defined Extracellular Matrix Cues and Morphogen Gradients in a Spindle-Shaped Microfluidic Device.Advanced healthcare materials · 2024Article
- Degradability tunes ECM stress relaxation and cellular mechanics.bioRxiv : the preprint server for biology · 2024Article
- Matrix confinement modulates 3D spheroid sorting and burst-like collective migration.Acta biomaterialia · 2024Article
119 more citing papers are in PubMed but not listed here.
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5 authors.
Funding
Abstract
Numerous studies have described the effects of matrix stiffening on cell behavior using two-dimensional synthetic surfaces; however, less is known about the effects of matrix stiffening on cells embedded in three-dimensional in vivo-like matrices. A primary limitation in investigating the effects of matrix stiffness in three dimensions is the lack of materials that can be tuned to control stiffness independently of matrix density. Here, we use collagen-based scaffolds where the mechanical properties are tuned using non-enzymatic glycation of the collagen in solution, prior to polymerization. Collagen solutions glycated prior to polymerization result in collagen gels with a threefold increase in compressive modulus without significant changes to the collagen architecture. Using these scaffolds, we show that endothelial cell spreading increases with matrix stiffness, as does the number and length of angiogenic sprouts and the overall spheroid outgrowth. Differences in sprout length are maintained even when the receptor for advanced glycation end products is inhibited. Our results demonstrate the ability to de-couple matrix stiffness from matrix density and structure in collagen gels, and that increased matrix stiffness results in increased sprouting and outgrowth.
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