ArticleThe Journal of biological chemistry2012
Proteomic analysis demonstrates activator- and chromatin-specific recruitment to promoters.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- A mechanism of synergistic Mediator recruitment in RNA polymerase II transcription activation revealed by single-molecule fluorescence.Molecular cell · 2025Article
- Single-molecule analysis of transcription activation: dynamics of SAGA coactivator recruitment.Nature structural & molecular biology · 2025Article
- A set of Saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteins.G3 (Bethesda, Md.) · 2022Article
- Gds1 Interacts with NuA4 To Promote H4 Acetylation at Ribosomal Protein Genes.Molecular and cellular biology · 2022Article
- PBRM1 loss in kidney cancer unbalances the proximal tubule master transcription factor hub to repress proximal tubule differentiation.Cell reports · 2021Article
- Dynamics of RNA polymerase II and elongation factor Spt4/5 recruitment during activator-dependent transcription.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- AMPK controls the axonal regenerative ability of dorsal root ganglia sensory neurons after spinal cord injury.Nature metabolism · 2020Article
- Targeting sickle cell disease root-cause pathophysiology with small molecules.Haematologica · 2019Review
- In vitro analysis of RNA polymerase II elongation complex dynamics.Genes & development · 2019Article
- In vitro assembly and proteomic analysis of RNA polymerase II complexes.Methods (San Diego, Calif.) · 2019Article
- SILAC-MS Profiling of Reconstituted Human Chromatin Platforms for the Study of Transcription and RNA Regulation.Journal of proteome research · 2018Article
- Transcription regulation by the Mediator complex.Nature reviews. Molecular cell biology · 2018Review
- Downstream promoter interactions of TFIID TAFs facilitate transcription reinitiation.Genes & development · 2017Article
- GATA4 loss of function in liver cancer impedes precursor to hepatocyte transition.The Journal of clinical investigation · 2017Article
- Mot1, Ino80C, and NC2 Function Coordinately to Regulate Pervasive Transcription in Yeast and Mammals.Molecular cell · 2017Article
- Discovery of Enzymatic Targets of Transcriptional Activators via in Vivo Covalent Chemical Capture.Journal of the American Chemical Society · 2016Article
- Heterochromatic gene silencing by activator interference and a transcription elongation barrier.The Journal of biological chemistry · 2013Article
- Dismantling promoter-driven RNA polymerase II transcription complexes in vitro by the termination factor Rat1.The Journal of biological chemistry · 2013Article
- The dynamics of HCF-1 modulation of herpes simplex virus chromatin during initiation of infection.Viruses · 2013Review
- Tailoring the models of transcription.International journal of molecular sciences · 2013Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
In-depth characterization of RNA polymerase II preinitiation complexes remains an important and challenging goal. We used quantitative mass spectrometry to explore context-dependent Saccharomyces cerevisiae preinitiation complex formation at the HIS4 promoter reconstituted on naked and chromatinized DNA templates. The transcription activators Gal4-VP16 and Gal4-Gcn4 recruited a limited set of chromatin-related coactivator complexes, namely the chromatin remodeler Swi/Snf and histone acetyltransferases SAGA and NuA4, suggesting that transcription stimulation is mediated through these factors. Moreover, the two activators differentially recruited the coactivator complexes, consistent with specific activator-coactivator interactions. Chromatinized templates suppressed recruitment of basal transcription factors, thereby amplifying the effect of activators, compared with naked DNA templates. This system is sensitive, highly reproducible, and easily applicable to mapping the repertoire of proteins found at any promoter.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.