ArticleCell2012
A genome-scale resource for in vivo tag-based protein function exploration in C. elegans.
Article in Cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 180 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
180 citing papers in PubMed.
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- Crossover patterning through condensation and coarsening of pro-crossover factors.Nature cell biology · 2025Article
- Transcription-templated assembly of the nucleolus in theProceedings of the National Academy of Sciences of the United States of America · 2025Article
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- CyanoTag: Discovery of protein function facilitated by high-throughput endogenous tagging in a photosynthetic prokaryote.Science advances · 2025Article
- Screening conditions and constructs for attempted genetic transformation of C. elegans by Agrobacterium.PloS one · 2025Article
- Binding profiles for 961Genome research · 2024Article
- Rapid and dynamic detection of endogenous proteins through in locus tagging in rice.Plant communications · 2024Article
- A neurotransmitter atlas ofeLife · 2024Article
- Recent Advancements in Subcellular Proteomics: Growing Impact of Organellar Protein Niches on the Understanding of Cell Biology.Journal of proteome research · 2024Review
- XOL-1 regulates developmental timing by modulating the H3K9 landscape in C. elegans early embryos.PLoS genetics · 2024Article
- Mitochondrial clearance and increased HSF-1 activity are coupled to promote longevity in fastediScience · 2024Article
- A neurotransmitter atlas ofbioRxiv : the preprint server for biology · 2024Article
- The Rac1 homolog CED-10 is a component of the MES-1/SRC-1 pathway for asymmetric division of thebioRxiv : the preprint server for biology · 2024Article
- A pair of RNA binding proteins inhibit ion transporter expression to maintain lifespan.Genetics · 2024Article
- Binding profiles for 954 Drosophila andbioRxiv : the preprint server for biology · 2024Article
120 more citing papers are in PubMed but not listed here.
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Authors and funding
27 authors.
Funding
Abstract
Understanding the in vivo dynamics of protein localization and their physical interactions is important for many problems in biology. To enable systematic protein function interrogation in a multicellular context, we built a genome-scale transgenic platform for in vivo expression of fluorescent- and affinity-tagged proteins in Caenorhabditis elegans under endogenous cis regulatory control. The platform combines computer-assisted transgene design, massively parallel DNA engineering, and next-generation sequencing to generate a resource of 14,637 genomic DNA transgenes, which covers 73% of the proteome. The multipurpose tag used allows any protein of interest to be localized in vivo or affinity purified using standard tag-based assays. We illustrate the utility of the resource by systematic chromatin immunopurification and automated 4D imaging, which produced detailed DNA binding and cell/tissue distribution maps for key transcription factor proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.