ArticleJournal of biological engineering2012
Development of a modified Respiration Activity Monitoring System for accurate and highly resolved measurement of respiration activity in shake flask fermentations.
Article in Journal of biological engineering, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Acetate-containing supernatants from industrial off-gas cultivation enabling high-value product formation with established and emerging production organisms.Biotechnology for biofuels and bioproducts · 2026Article
- Online Monitoring of Oxygen Transfer Rate as a Versatile Tool for Fermentation Characterization and Optimization.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Sampling-free investigation of microbial carbon source preferences on renewable feedstocks via online monitoring of oxygen transfer rate.Bioprocess and biosystems engineering · 2025Article
- A new approach to off-gas analysis for shaken bioreactors showing high CTR and RQ accuracy.Journal of biological engineering · 2025Article
- A forced aeration system for microbial culture of multiple shaken vessels suppresses volatilization.Archives of microbiology · 2024Article
- Improved Time Resolved KPI and Strain Characterization of Multiple Hosts in Shake Flasks Using Advanced Online Analytics and Data Science.Bioengineering (Basel, Switzerland) · 2022Article
- Optimization of the production process for the anticancer lead compound illudin M: process development in stirred tank bioreactors.Microbial cell factories · 2022Article
- Optimization of the production process for the anticancer lead compound illudin M: improving titers in shake-flasks.Microbial cell factories · 2022Article
- Implementation of Perforated Concentric Ring Walls Considerably Improves Gas-Liquid Mass Transfer of Shaken Bioreactors.Frontiers in bioengineering and biotechnology · 2022Article
- Time-Resolved Monitoring of the Oxygen Transfer Rate of Chinese Hamster Ovary Cells Provides Insights Into Culture Behavior in Shake Flasks.Frontiers in bioengineering and biotechnology · 2021Article
- Real-time dissolved carbon dioxide monitoring I: Application of a novel in situ sensor for COBiotechnology and bioengineering · 2020Article
- Article
- The metabolic switch can be activated in a recombinant strain of Streptomyces lividans by a low oxygen transfer rate in shake flasks.Microbial cell factories · 2018Article
- Easy to use and reliable technique for online dissolved oxygen tension measurement in shake flasks using infrared fluorescent oxygen-sensitive nanoparticles.Microbial cell factories · 2016Article
- Online monitoring of dissolved oxygen tension in microtiter plates based on infrared fluorescent oxygen-sensitive nanoparticles.Microbial cell factories · 2015Article
- A particular silent codon exchange in a recombinant gene greatly influences host cell metabolic activity.Microbial cell factories · 2015Article
- Parallel use of shake flask and microtiter plate online measuring devices (RAMOS and BioLector) reduces the number of experiments in laboratory-scale stirred tank bioreactors.Journal of biological engineering · 2015Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe Respiration Activity Monitoring System (RAMOS) is an established device to measure on-line the oxygen transfer rate (OTR), thereby, yielding relevant information about metabolic activities of microorganisms and cells during shake flask fermentations. For very fast-growing microbes, however, the RAMOS technique provides too few data points for the OTR. Thus, this current study presents a new model based evaluation method for generating much more data points to enhance the information content and the precision of OTR measurements.
resultsIn cultivations with E.coli BL21 pRSET eYFP-IL6, short diauxic and even triauxic metabolic activities were detected with much more detail compared to the conventional evaluation method. The decline of the OTR during the stop phases during oxygen limitations, which occur when the inlet and outlet valves of the RAMOS flask were closed for calibrating the oxygen sensor, were also detected. These declines reflected a reduced oxygen transfer due to the stop phases. In contrast to the conventional calculation method the new method was almost independent from the number of stop phases chosen in the experiments.
conclusionsThis new model based evaluation method unveils new peaks of metabolic activity which otherwise would not have been resolved by the conventional RAMOS evaluation method. The new method yields substantially more OTR data points, thereby, enhancing the information content and the precision of the OTR measurements. Furthermore, oxygen limitations can be detected by a decrease of the OTR during the stop phases.
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