ArticleBioconjugate chemistry2012
Caspase-activated cell-penetrating peptides reveal temporal coupling between endosomal release and apoptosis in an RGC-5 cell model.
Article in Bioconjugate chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
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- Intracellular Delivery of Therapeutic Protein via Ultrathin Layered Double Hydroxide Nanosheets.Pharmaceutics · 2024Article
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- Enhancing Cell Penetration Efficiency of Cyclic Oligoarginines Using Rigid Scaffolds.Pharmaceutics · 2023Article
- Cell-Penetrating Dabcyl-Containing Tetraarginines with Backbone Aromatics as Uptake Enhancers.Pharmaceutics · 2022Article
- Apoptosis Detection in Retinal Ganglion Cells Using Quantitative Changes in Multichannel Fluorescence Colocalization.Biosensors · 2022Article
- Bimodal fluorogenic sensing of matrix proteolytic signatures in lung cancer.Organic & biomolecular chemistry · 2018Article
- Protein delivery into cells using inorganic nanoparticle-protein supramolecular assemblies.Chemical Society reviews · 2018Review
- Intracellular delivery of proteins by nanocarriers.Nanomedicine (London, England) · 2017Review
- Eliminating caspase-7 and cathepsin B cross-reactivity on fluorogenic caspase-3 substrates.Molecular bioSystems · 2016Article
- MMP-2/9-Specific Activatable Lifetime Imaging Agent.Sensors (Basel, Switzerland) · 2015Article
- Caspase-responsive smart gadolinium-based contrast agent for magnetic resonance imaging of drug-induced apoptosis.Chemical science · 2014Article
- Promises and pitfalls of intracellular delivery of proteins.Bioconjugate chemistry · 2014Review
- Protein delivery into live cells by incubation with an endosomolytic agent.Nature methods · 2014Article
- Evaluating new therapies in gastrointestinal stromal tumor using in vivo molecular optical imaging.Cancer biology & therapy · 2014Article
- Bioorthogonal cyclization-mediated in situ self-assembly of small-molecule probes for imaging caspase activity in vivo.Nature chemistry · 2014Article
- Single-cell resolution imaging of retinal ganglion cell apoptosis in vivo using a cell-penetrating caspase-activatable peptide probe.PloS one · 2014Article
- Improved assays for determining the cytosolic access of peptides, proteins, and their mimetics.Biochemistry · 2013Article
- Image-Guided Tumor Resection.Cancer journal (Sudbury, Mass.)Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Caspase-activatable cell-penetrating peptide (CPP) probes, designed for efficient cell uptake and specificity via cleavable intramolecular quenched-fluorophore strategies, show promise for identifying and imaging retinal ganglion cell apoptosis in vivo. However, initial cell uptake and trafficking events cannot be visualized because the probes are designed to be optically quenched in the intact state. To visualize subcellular activation events in real-time during apoptosis, a new series of matched quenched and nonquenched CPP probes were synthesized. In both native and staurosporine-differentiated RGC-5 cells, probe uptake was time- and concentration-dependent through clathrine-, caveolin-, and pinocytosis-mediated endocytic mechanisms. During apoptosis, KcapTR488, a novel dual fluorophore CPP probe, revealed by multispectral imaging a temporal coupling of endosomal release and effector caspase activation in RGC-5 cells. The novel CPPs described herein provide new tools to study spatial and temporal regulation of endosomal permeability during apoptosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.