ArticlePloS one2012
Functional characterizations of chemosensory proteins of the alfalfa plant bug Adelphocoris lineolatus indicate their involvement in host recognition.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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61 citing papers in PubMed, 129 citations in OpenAlex.
- Sequence and structure analysis for chemosensory proteins and odorant-binding proteins in Nilaparvata lugens (Hemiptera: Delphacidae).Journal of insect science (Online) · 2026Article
- The Odorant-Binding Proteins AspiOBP1 and AspiOBP2 inInternational journal of molecular sciences · 2025Article
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- Identification, expression profiles, and binding properties of chemosensory protein 18 in Plutella xylostella (Lepidoptera: Plutellidae).Journal of insect science (Online) · 2024Article
- Expression Pattern and Ligand Binding Characteristics Analysis of Chemosensory Protein SnitCSP2 fromInsects · 2023Article
- Data-driven design of a multiplexed, peptide-sensitized transistor to detect breath VOC markers of COVID-19.Biosensors & bioelectronics · 2023Article
- Odorant-Binding Proteins and Chemosensory Proteins inInternational journal of molecular sciences · 2023Article
- The Antennal Sensilla and Expression Patterns of Olfactory Genes in the Lower Termite Reticulitermes aculabialis (Isoptera: Rhinotermitidae).Journal of insect science (Online) · 2022Article
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- Transcriptome analysis ofFrontiers in physiology · 2022Article
- Genome-Wide Analysis of Odorant-Binding Proteins and Chemosensory Proteins in the Bean bugFrontiers in physiology · 2022Article
- Transcriptomic analysis of resistance and short-term induction response to pyrethroids, in Anopheles coluzzii legs.BMC genomics · 2021Article
- Antennal transcriptome sequencing and identification of candidate chemoreceptor proteins from an invasive pest, the American palm weevil, Rhynchophorus palmarum.Scientific reports · 2021Article
- Expression Profiles and Functional Characterization of Chemosensory Protein 15 (HhalCSP15) in the Brown Marmorated Stink BugFrontiers in physiology · 2021Article
- Article
- Expression Profiles and Binding Properties of the Chemosensory Protein PxylCSP11 from the Diamondback Moth, Plutella xylostella (Lepidoptera: Plutellidae).Journal of insect science (Online) · 2020Article
- Red Imported Fire Ant (Frontiers in physiology · 2020Article
- A Chemosensory ProteinFrontiers in physiology · 2020Article
- Chemosensory Gene Families in the Oligophagous Pear PestInsects · 2019Article
- Article
1 more citing papers are in PubMed but not listed here.
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10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Insect chemosensory proteins (CSPs) have been proposed to capture and transport hydrophobic chemicals from air to olfactory receptors in the lymph of antennal chemosensilla. They may represent a new class of soluble carrier protein involved in insect chemoreception. However, their specific functional roles in insect chemoreception have not been fully elucidated. In this study, we report for the first time three novel CSP genes (AlinCSP1-3) of the alfalfa plant bug Adelphocoris lineolatus (Goeze) by screening the antennal cDNA library. The qRT-PCR examinations of the transcript levels revealed that all three genes (AlinCSP1-3) are mainly expressed in the antennae. Interestingly, these CSP genes AlinCSP1-3 are also highly expressed in the 5(th) instar nymphs, suggesting a proposed function of these CSP proteins (AlinCSP1-3) in the olfactory reception and in maintaining particular life activities into the adult stage. Using bacterial expression system, the three CSP proteins were expressed and purified. For the first time we characterized the types of sensilla in the antennae of the plant bug using scanning electron microscopy (SEM). Immunocytochemistry analysis indicated that the CSP proteins were expressed in the pheromone-sensitive sensilla trichodea and general odorant-sensitive sensilla basiconica, providing further evidence of their involvement in chemoreception. The antennal activity of 55 host-related semiochemicals and sex pheromone compounds in the host location and mate selection behavior of A. lineolatus was investigated using electroantennogram (EAG), and the binding affinities of these chemicals to the three CSPs (AlinCSP1-3) were measured using fluorescent binding assays. The results showed several host-related semiochemicals, (Z)-3-hexen-1-ol, (E)-2-hexen-1-al and valeraldehyde, have a high binding affinity with AlinCSP1-3 and can elicit significant high EAG responses of A. lineolatus antennae. Our studies indicate the three antennae-biased CSPs may mediate host recognition in the alfalfa plant bug A. lineolatus.
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