ArticlePloS one2012
Visualization of Src and FAK activity during the differentiation process from HMSCs to osteoblasts.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- A mechanosensitive circuit of FAK, ROCK, and ERK controls biomineral growth and morphology in the sea urchin embryo.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Integrating network pharmacology, IPA, and molecular docking to reveal the anti-osteoporosis effects of EA and EB via the FAK pathway.Frontiers in pharmacology · 2025Article
- Targeted activation of androgen receptor signaling in the periosteum improves bone fracture repair.Cell death & disease · 2022Article
- Effects of PIN on Osteoblast Differentiation and Matrix Mineralization through Runt-Related Transcription Factor.International journal of molecular sciences · 2020Article
- 7-HYB, a Phenolic Compound Isolated fromInternational journal of molecular sciences · 2020Article
- TMARg, a Novel Anthraquinone Isolated fromInternational journal of molecular sciences · 2020Article
- A Phytochemical Constituent, (E)-Methyl-Cinnamate Isolated fromInternational journal of molecular sciences · 2020Article
- Article
- SRC kinase inhibition with saracatinib limits the development of osteolytic bone disease in multiple myeloma.Oncotarget · 2016Article
- Wnt/β-catenin signaling mediates osteoblast differentiation triggered by peptide-induced α5β1 integrin priming in mesenchymal skeletal cells.The Journal of biological chemistry · 2015Article
- Single-cell imaging of mechanotransduction in endothelial cells.Progress in molecular biology and translational science · 2014Review
- CD13 promotes mesenchymal stem cell-mediated regeneration of ischemic muscle.Frontiers in physiology · 2014Article
- The effect of differentiation induction on FAK and Src activity in live HMSCs visualized by FRET.PloS one · 2013Article
- Sonic hedgehog regulates osteoblast function by focal adhesion kinase signaling in the process of fracture healing.PloS one · 2013Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Non-receptor protein kinases FAK and Src play crucial roles in regulating cellular adhesions, growth, migration and differentiation. However, it remains unclear how the activity of FAK and Src is regulated during the differentiation process from mesenchymal stem cells (MSCs) to bone cells. In this study, we used genetically encoded FAK and Src biosensors based on fluorescence resonance energy transfer (FRET) to monitor the FAK and Src activity in live cells during the differentiation process. The results revealed that the FAK activity increased after the induction of differentiation, which peaked around 20-27 days after induction. Meanwhile, the Src activity decreased continuously for 27 days after induction. Therefore, the results showed significant and differential changes of FAK and Src activity upon induction. This opposite trend between FAK and Src activation suggests novel and un-coupled Src/FAK functions during the osteoblastic differentiation process. These results should provide important information for the biochemical signals during the differentiation process of stem cells toward bone cells, which will advance our understanding of bone repair and tissue engineering.
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Registered trials
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