ArticlePloS one2012
Nuclear import and dimerization of tomato ASR1, a water stress-inducible protein exclusive to plants.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 32 citations in OpenAlex.
- Cloning and functional determination of PpyASR1 during fruit development in Pyrus pyrifolia.BMC plant biology · 2026Article
- Self-association and multimer formation in AtLEA4-5, a desiccation-induced intrinsically disordered protein from plants.Protein science : a publication of the Protein Society · 2024Article
- Abscisic acid-, stress-, ripening-induced 2 like protein, TaASR2L, promotes wheat resistance to stripe rust.Molecular plant pathology · 2024Article
- Grape ASR Regulates Glucose Transport, Metabolism and Signaling.International journal of molecular sciences · 2022Article
- Article
- Grape ASR-Silencing Sways Nuclear Proteome, Histone Marks and Interplay of Intrinsically Disordered Proteins.International journal of molecular sciences · 2022Article
- Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato.PloS one · 2019Article
- Conformational plasticity of the intrinsically disordered protein ASR1 modulates its function as a drought stress-responsive gene.PloS one · 2018Article
- OsASR5 enhances drought tolerance through a stomatal closure pathway associated with ABA and HPlant biotechnology journal · 2017Article
- Molecular cloning and characterization of drought stress responsive abscisic acid-stress-ripening (Asr 1) gene from wild jujube, Ziziphus nummularia (Burm.f.) Wight & Arn.Molecular biology reports · 2016Article
- AUXIN RESPONSE FACTOR 2 Intersects Hormonal Signals in the Regulation of Tomato Fruit Ripening.PLoS genetics · 2016Article
- The MaASR gene as a crucial component in multiple drought stress response pathways in Arabidopsis.Functional & integrative genomics · 2015Article
- Nuclear localization of the dehydrin OpsDHN1 is determined by histidine-rich motif.Frontiers in plant science · 2015Article
- Insights into transcriptional regulation of β-D-N-acetylhexosaminidase, an N-glycan-processing enzyme involved in ripening-associated fruit softening.Journal of experimental botany · 2014Article
- Review
- New insights into aluminum tolerance in rice: the ASR5 protein binds the STAR1 promoter and other aluminum-responsive genes.Molecular plant · 2014Article
- Article
- Epigenetic marks in an adaptive water stress-responsive gene in tomato roots under normal and drought conditions.Epigenetics · 2013Article
- Abiotic stress responsive rice ASR1 and ASR3 exhibit different tissue-dependent sugar and hormone-sensitivities.Molecules and cells · 2013Article
- ASR1 mediates glucose-hormone cross talk by affecting sugar trafficking in tobacco plants.Plant physiology · 2013Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ASR (for ABA/water stress/ripening) protein family, first described in tomato as nuclear and involved in adaptation to dry climates, is widespread in the plant kingdom, including crops of high agronomic relevance. We show both nuclear and cytosolic localization for ASR1 (the most studied member of the family) in histological plant samples by immunodetection, typically found in small proteins readily diffusing through nuclear pores. Indeed, a nuclear localization was expected based on sorting prediction software, which also highlight a monopartite nuclear localization signal (NLS) in the primary sequence. However, here we prove that such an "NLS" of ASR1 from tomato is dispensable and non-functional, being the transport of the protein to the nucleus due to simple diffusion across nuclear pores. We attribute such a targeting deficiency to the misplacing in that cryptic NLS of two conserved contiguous lysine residues. Based on previous in vitro experiments regarding quaternary structure, we also carried out live cell imaging assays through confocal microscopy to explore dimer formation in planta. We found homodimers in both the cytosol and the nucleus and demonstrated that assembly of both subunits together can occur in the cytosol, giving rise to translocation of preformed dimers. The presence of dimers was further corroborated by means of in vivo crosslinking of nuclei followed by SDS-PAGE.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.