ArticleMolecular & cellular proteomics : MCP2012
A deep exploration of the transcriptome and "excretory/secretory" proteome of adult Fascioloides magna.
Article in Molecular & cellular proteomics : MCP, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 36 citations in OpenAlex.
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- Protein profile of extracellular vesicles derived from adult Parascaris spp.Parasites & vectors · 2024Article
- Liver Proteome Alterations in Red Deer (Pathogens (Basel, Switzerland) · 2022Article
- Evidence of Immune Modulators in the Secretome of the Equine TapewormPathogens (Basel, Switzerland) · 2021Article
- Eudiplozoon nipponicum (Monogenea, Diplozoidae) and its adaptation to haematophagy as revealed by transcriptome and secretome profiling.BMC genomics · 2021Article
- Evolutionary Analysis of Cystatins of Early-Emerging Metazoans Reveals a Novel Subtype in Parasitic Cnidarians.Biology · 2021Article
- Molecular signatures of the rediae, cercariae and adult stages in the complex life cycles of parasitic flatworms (Digenea: Psilostomatidae).Parasites & vectors · 2020Article
- Secreted cathepsin L-like peptidases are involved in the degradation of trapped antibodies on the surface of Echinostoma caproni.Parasitology research · 2019Article
- Polyomic tools for an emerging livestock parasite, the rumen fluke Calicophoron daubneyi; identifying shifts in rumen functionality.Parasites & vectors · 2018Article
- Immunoproteomics and Surfaceomics of the Adult TapewormFrontiers in immunology · 2018Article
- De novo genome and transcriptome analyses provide insights into the biology of the trematode human parasite Fasciolopsis buski.PloS one · 2018Article
- Conservation and diversification of the transcriptomes of adult Paragonimus westermani and P. skrjabini.Parasites & vectors · 2016Article
- Complete mitochondrial genome of the giant liver fluke Fascioloides magna (Digenea: Fasciolidae) and its comparison with selected trematodes.Parasites & vectors · 2016Article
- The omic approach to parasitic trematode research-a review of techniques and developments within the past 5 years.Parasitology research · 2016Review
- De novo transcriptomic analysis of the female and male adults of the blood fluke Schistosoma turkestanicum.Parasites & vectors · 2016Article
- Identification and characterization of Clonorchis sinensis cathepsin B proteases in the pathogenesis of clonorchiasis.Parasites & vectors · 2015Article
- Functional expression of a novel Kunitz type protease inhibitor from the human blood fluke Schistosoma mansoni.Parasites & vectors · 2015Article
- Transcriptome and venom proteome of the box jellyfish Chironex fleckeri.BMC genomics · 2015Article
- Nuclear genomic signals of the 'microturbellarian' roots of platyhelminth evolutionary innovation.eLife · 2015Article
- Transcriptome profiles of the protoscoleces of Echinococcus granulosus reveal that excretory-secretory products are essential to metabolic adaptation.PLoS neglected tropical diseases · 2014Article
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Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Parasitic liver flukes of the family Fasciolidae are responsible for major socioeconomic losses worldwide. However, at present, knowledge of the fundamental molecular biology of these organisms is scant. Here, we characterize, for the first time, the transcriptome and secreted proteome of the adult stage of the "giant liver fluke," Fascioloides magna, using Illumina sequencing technology and one-dimensional SDS-PAGE and OFFGEL protein electrophoresis, respectively. A total of ∼54,000,000 reads were generated and assembled into ∼39,000 contiguous sequences (contigs); ∼20,000 peptides were predicted and classified based on homology searches, protein motifs, gene ontology, and biological pathway mapping. From the predicted proteome, 48.1% of proteins could be assigned to 384 biological pathway terms, including "spliceosome," "RNA transport," and "endocytosis." Putative proteins involved in amino acid degradation were most abundant. Of the 835 secreted proteins predicted from the transcriptome of F. magna, 80 were identified in the excretory/secretory products from this parasite. Highly represented were antioxidant proteins, followed by peptidases (particularly cathepsins) and proteins involved in carbohydrate metabolism. The integration of transcriptomic and proteomic datasets generated herein sets the scene for future studies aimed at exploring the potential role(s) that molecules might play at the host-parasite interface and for establishing novel strategies for the treatment or control of parasitic fluke infections.
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