ArticleJournal of cell science2012
Filopodium retraction is controlled by adhesion to its tip.
Article in Journal of cell science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 46 citations in OpenAlex.
- Modeling the roles of cohesotaxis, cell-intercalation, and tissue geometry in collective cell migration of Xenopus mesendoderm.Biology open · 2024Article
- Rho-associated kinase regulates Langerhans cell morphology and responsiveness to tissue damage.Cell reports · 2024Article
- Mechanisms underlying Myosin 10's contribution to the maintenance of mitotic spindle bipolarity.Molecular biology of the cell · 2024Article
- Measuring pico-Newton Forces with Lipid Anchors as Force Sensors in Molecular Dynamics Simulations.The journal of physical chemistry. B · 2023Article
- Filopodia rotate and coil by actively generating twist in their actin shaft.Nature communications · 2022Article
- Vibration, a treatment for migraine, linked to calpain driven changes in actin cytoskeleton.PloS one · 2022Article
- Mechanotransduction as a major driver of cell behaviour: mechanisms, and relevance to cell organization and future research.Open biology · 2021Review
- Natural Fish Trap-Like Nanocage for Label-Free Capture of Circulating Tumor Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Actin Assembly around the Shigella-Containing Vacuole Promotes Successful Infection.Cell reports · 2020Article
- Myosin IIA and formin dependent mechanosensitivity of filopodia adhesion.Nature communications · 2019Article
- Review
- ROS induced distribution of mitochondria to filopodia by Myo19 depends on a class specific tryptophan in the motor domain.Scientific reports · 2017Article
- Membrane curvature regulates ligand-specific membrane sorting of GPCRs in living cells.Nature chemical biology · 2017Article
- Semiflexible Biopolymers in Bundled Arrangements.Polymers · 2016Review
- Surfing along Filopodia: A Particle Transport Revealed by Molecular-Scale Fluctuation Analyses.Biophysical journal · 2015Article
- Helical buckling of actin inside filopodia generates traction.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Filopodial retraction force is generated by cortical actin dynamics and controlled by reversible tethering at the tip.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The inside story of Shigella invasion of intestinal epithelial cells.Cold Spring Harbor perspectives in medicine · 2013Review
- Adenosine-A3 receptors in neutrophil microdomains promote the formation of bacteria-tethering cytonemes.EMBO reports · 2013Article
- Cell signaling experiments driven by optical manipulation.International journal of molecular sciences · 2013Review
Corrections and comments
- Erratum issuedBornschlög, Thomas [corrected to Bornschlögl, Thomas]
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Filopodia are thin cell extensions sensing the environment. They play an essential role during cell migration, cell-cell or cell-matrix adhesion, by initiating contacts and conveying signals to the cell cortex. Pathogenic microorganisms can hijack filopodia to invade cells by inducing their retraction towards the cell body. Because their dynamics depend on a discrete number of actin filaments, filopodia provide a model of choice to study elementary events linked to adhesion and downstream signalling. However, the determinants controlling filopodial sensing are not well characterized. In this study, we used beads functionalized with different ligands that triggered filopodial retraction when in contact with filopodia of epithelial cells. With optical tweezers, we were able to measure forces stalling the retraction of a single filopodium. We found that the filopodial stall force depends on the coating of the bead. Stall forces reached 8 pN for beads coated with the β1 integrin ligand Yersinia Invasin, whereas retraction was stopped with a higher force of 15 pN when beads were functionalized with carboxyl groups. In all cases, stall forces increased in relation to the density of ligands contacting filopodial tips and were independent of the optical trap stiffness. Unexpectedly, a discrete and small number of Shigella type three secretion systems induced stall forces of 10 pN. These results suggest that the number of receptor-ligand interactions at the filopodial tip determines the maximal retraction force exerted by filopodia but a discrete number of clustered receptors is sufficient to induce high retraction stall forces.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.