ArticleNature protocols2012
Radiometric and spectrophotometric in vitro assays of glycosyltransferases involved in plant cell wall carbohydrate biosynthesis.
Article in Nature protocols, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 41 citations in OpenAlex.
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- Fit-for-purpose heterodivalent single-domain antibody for gastrointestinal targeting of toxin B from Clostridium difficile.Protein science : a publication of the Protein Society · 2024Article
- The plant cell wall-dynamic, strong, and adaptable-is a natural shapeshifter.The Plant cell · 2024Review
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- The Gram-positive bacterium Romboutsia ilealis harbors a polysaccharide synthase that can produce (1,3;1,4)-β-D-glucans.Nature communications · 2023Article
- Genome-wide identification and expression analysis of GDP-D-mannose pyrophosphorylase and KATANIN inFrontiers in plant science · 2023Article
- Molecular studies of cellulose synthase supercomplex from cotton fiber reveal its unique biochemical properties.Science China. Life sciences · 2022Article
- Endosidin20 Targets the Cellulose Synthase Catalytic Domain to Inhibit Cellulose Biosynthesis.The Plant cell · 2020Article
- The glycosyltransferase UGT76E1 significantly contributes to 12-The Journal of biological chemistry · 2019Article
- Cellulose Synthase Stoichiometry in Aspen Differs from Arabidopsis and Norway Spruce.Plant physiology · 2018Article
- Proteomic Analysis of Plasmodesmata FromFrontiers in plant science · 2018Article
- A single heterologously expressed plant cellulose synthase isoform is sufficient for cellulose microfibril formation in vitro.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Article
- Bacterial Glycosyltransferases: Challenges and Opportunities of a Highly Diverse Enzyme Class Toward Tailoring Natural Products.Frontiers in microbiology · 2016Article
- MUCILAGE-RELATED10 Produces Galactoglucomannan That Maintains Pectin and Cellulose Architecture in Arabidopsis Seed Mucilage.Plant physiology · 2015Article
- A molecular description of cellulose biosynthesis.Annual review of biochemistry · 2015Review
- Mechanism of activation of bacterial cellulose synthase by cyclic di-GMP.Nature structural & molecular biology · 2014Article
- BcsA and BcsB form the catalytically active core of bacterial cellulose synthase sufficient for in vitro cellulose synthesis.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most of the glycosyltransferases (GTs) that catalyze the formation of plant cell wall carbohydrates remain to be biochemically characterized. This can be achieved only if specific assays are available for these enzymes. Here we present a protocol for in vitro assays of processive and nonprocessive membrane-bound GTs. The assays are either based on the use of radioactive nucleotide sugars (NDP sugars; e.g., UDP-[U-(14)C]glucose) and the quantification of the radiolabeled monosaccharides incorporated into soluble or insoluble carbohydrates, or on the coupling of the GT reaction with that of pyruvate kinase (PK) and the oxidation of NADH by lactate dehydrogenase (LDH). The radiometric assays are more suitable for exploratory work on poorly characterized enzymes, whereas the spectrophotometric assays require the availability of highly enriched GTs. Both assays can be performed within 1 d, depending on the number of fractions to be assayed or reaction mixtures to be tested.
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