ArticleNature protocols2012
Surface functionalization of barium titanate SHG nanoprobes for in vivo imaging in zebrafish.
Article in Nature protocols, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 79 citations in OpenAlex.
- Exploring the piezoelectric phenomenon: From polymers to human tissues and advanced applications in tissue engineering.Bioactive materials · 2026Review
- Piezoelectric biomaterials for providing electrical stimulation in bone tissue engineering: Barium titanate.Journal of orthopaedic translation · 2025Review
- A gene-encoded bioprotein second harmonic generation (SHG) probe from Autographa californica nuclear polyhedrosis virus (AcMNPV) polyhedrin for live cell imaging.European biophysics journal : EBJ · 2025Article
- Nanoparticulate Perovskites for Photocatalytic Water Reduction.Nanomaterials (Basel, Switzerland) · 2023Article
- Biodegradable Harmonophores for Targeted High-ResolutionACS nano · 2021Article
- Comparison of Methods for Surface Modification of Barium Titanate Nanoparticles for Aqueous Dispersibility: Toward Biomedical Utilization of Perovskite Oxides.ACS applied materials & interfaces · 2020Article
- FastACS photonics · 2020Article
- Antibody-Conjugated Barium Titanate Nanoparticles for Cell-Specific Targeting.ACS applied nano materials · 2020Article
- Advancing Versatile Ferroelectric Materials Toward Biomedical Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Review
- Piezoelectric barium titanate nanostimulators for the treatment of glioblastoma multiforme.Journal of colloid and interface science · 2019Article
- BaTiOActa biomaterialia · 2018Article
- Confocal imaging of single BaTiOScientific reports · 2017Article
- Labeling cellular structures in vivo using confined primed conversion of photoconvertible fluorescent proteins.Nature protocols · 2016Article
- Determination of the source of SHG verniers in zebrafish skeletal muscle.Scientific reports · 2015Article
- Second harmonic super-resolution microscopy for quantification of mRNA at single copy sensitivity.ACS nano · 2014Article
- Advances in whole-embryo imaging: a quantitative transition is underway.Nature reviews. Molecular cell biology · 2014Review
- Automated processing of zebrafish imaging data: a survey.Zebrafish · 2013Review
- Characterization of the nonlinear optical properties of nanocrystals by Hyper Rayleigh Scattering.Journal of nanobiotechnology · 2013Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To address the need for a bright, photostable labeling tool that allows long-term in vivo imaging in whole organisms, we recently introduced second harmonic generating (SHG) nanoprobes. Here we present a protocol for the preparation and use of a particular SHG nanoprobe label, barium titanate (BT), for in vivo imaging in living zebrafish embryos. Chemical treatment of the BT nanoparticles results in surface coating with amine-terminal groups, which act as a platform for a variety of chemical modifications for biological applications. Here we describe cross-linking of BT to a biotin-linked moiety using click chemistry methods and coating of BT with nonreactive poly(ethylene glycol) (PEG). We also provide details for injecting PEG-coated SHG nanoprobes into zygote-stage zebrafish embryos, and in vivo imaging of SHG nanoprobes during gastrulation and segmentation. Implementing the PROCEDURE requires a basic understanding of laser-scanning microscopy, experience with handling zebrafish embryos and chemistry laboratory experience. Functionalization of the SHG nanoprobes takes ∼3 d, whereas zebrafish preparation, injection and imaging setup should take approximately 2-4 h.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.