ArticleBasic research in cardiology2012
Secretome of apoptotic peripheral blood cells (APOSEC) attenuates microvascular obstruction in a porcine closed chest reperfused acute myocardial infarction model: role of platelet aggregation and vasodilation.
Article in Basic research in cardiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 42 citations in OpenAlex.
- Thrombectomy in acute myocardial infarction: Current evidence, challenges, and emerging technologies.World journal of clinical cases · 2025Review
- The Secretome of Irradiated Peripheral Mononuclear Cells Attenuates Hypertrophic Skin Scarring.Pharmaceutics · 2023Article
- Paracrine Factors of Stressed Peripheral Blood Mononuclear Cells Activate Proangiogenic and Anti-Proteolytic Processes in Whole Blood Cells and Protect the Endothelial Barrier.Pharmaceutics · 2022Article
- PBMNCs Treatment in Critical Limb Ischemia and Candidate Biomarkers of Efficacy.Diagnostics (Basel, Switzerland) · 2022Review
- Article
- Comparing the efficacy of γ- and electron-irradiation of PBMCs to promote secretion of paracrine, regenerative factors.Molecular therapy. Methods & clinical development · 2021Article
- Article
- Apoptotic Cells for Therapeutic Use in Cytokine Storm Associated With Sepsis- A Phase Ib Clinical Trial.Frontiers in immunology · 2021Article
- The secretome of stressed peripheral blood mononuclear cells increases tissue survival in a rodent epigastric flap model.Bioengineering & translational medicine · 2021Article
- Role of the CXCR4-SDF1-HMGB1 pathway in the directional migration of cells and regeneration of affected organs.World journal of stem cells · 2020Review
- Quantitative Hybrid Cardiac [Molecular therapy. Methods & clinical development · 2020Article
- Role for Lipids Secreted by Irradiated Peripheral Blood Mononuclear Cells in Inflammatory Resolution in Vitro.International journal of molecular sciences · 2020Article
- Autologous serum supplement favours in vitro regenerative paracrine factors synthesis.Cell proliferation · 2017Article
- Ionizing radiation regulates long non-coding RNAs in human peripheral blood mononuclear cells.Journal of radiation research · 2017Article
- Peripheral blood mononuclear cell secretome for tissue repair.Apoptosis : an international journal on programmed cell death · 2016Review
- Dying blood mononuclear cell secretome exerts antimicrobial activity.European journal of clinical investigation · 2016Article
- Analysis of the Secretome of Apoptotic Peripheral Blood Mononuclear Cells: Impact of Released Proteins and Exosomes for Tissue Regeneration.Scientific reports · 2015Article
- Mononuclear cell secretome protects from experimental autoimmune myocarditis.European heart journal · 2015Article
- High dose ionizing radiation regulates micro RNA and gene expression changes in human peripheral blood mononuclear cells.BMC genomics · 2014Article
- Secretomes of apoptotic mononuclear cells ameliorate neurological damage in rats with focal ischemia.F1000Research · 2014Article
Corrections and comments
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Authors and funding
15 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although epicardial blood flow can be restored by an early intervention in most cases, a lack of adequate reperfusion at the microvascular level is often a limiting prognostic factor of acute myocardial infarction (AMI). Our group has recently found that paracrine factors secreted from apoptotic peripheral blood mononuclear cells (APOSEC) attenuate the extent of myocardial injury. The aim of this study was to determine the influence of APOSEC on microvascular obstruction (MVO) in a porcine AMI model. A single dose of APOSEC was intravenously injected in a closed chest reperfused infarction model. MVO was determined by magnetic resonance imaging and cardiac catheterization. Role of platelet function and vasodilation were monitored by means of ELISA, flow cytometry, aggregometry, western blot and myographic experiments in vitro and in vivo. Treatment of AMI with APOSEC resulted in a significant reduction of MVO. Platelet activation markers were reduced in plasma samples obtained during AMI, suggesting an anti-aggregatory capacity of APOSEC. This finding was confirmed by in vitro tests showing that activation and aggregation of both porcine and human platelets were significantly impaired by co-incubation with APOSEC, paralleled by vasodilator-stimulated phosphoprotein (VASP)-mediated inhibition of platelets. In addition, APOSEC evidenced a significant vasodilatory capacity on coronary arteries via p-eNOS and iNOS activation. Our data give first evidence that APOSEC reduces the extent of MVO during AMI, and suggest that modulation of platelet activation and vasodilation in the initial phase after myocardial infarction contributes to the improved long-term outcome in APOSEC treated animals.
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