ArticleProtein science : a publication of the Protein Society2012
Bacterial collagen-binding domain targets undertwisted regions of collagen.
Article in Protein science : a publication of the Protein Society, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 27 citations in OpenAlex.
- Bacterial collagenase harnesses collagen geometry for processive cleavage.Nature communications · 2026Article
- Engineering a dysbiotic biofilm model for testing root caries interventions through microbial modulation.Microbiome · 2024Article
- Overview of Bacterial Protein Toxins from Pathogenic Bacteria: Mode of Action and Insights into Evolution.Toxins · 2024Review
- The action of microbial collagenases in dentinal matrix degradation in root caries and potential strategies for its management: a comprehensive state-of-the-art review.Journal of applied oral science : revista FOB · 2024Review
- Effect of Different Crosslinkers on Denatured Dentin Collagen's Biostability, MMP Inhibition and Mechanical Properties.Polymers · 2023Article
- Salivary esterases dramatically reduce biostability of dentin collagen treated with galloylated polyphenols.Dental materials : official publication of the Academy of Dental Materials · 2022Article
- Streptococcus pneumoniae binds collagens and C1q via the SSURE repeats of the PfbB adhesin.Molecular microbiology · 2022Article
- Mechanistic Insight into the Fragmentation of Type I Collagen Fibers into Peptides and Amino Acids by aApplied and environmental microbiology · 2022Article
- Structure of Vibrio collagenase VhaC provides insight into the mechanism of bacterial collagenolysis.Nature communications · 2022Article
- Distinct effects of polyphenols and solvents on dentin collagen crosslinking interactions and biostability.Dental materials : official publication of the Academy of Dental Materials · 2021Article
- The C-terminal segment of collagenase inFEBS open bio · 2018Article
- CaThe FEBS journal · 2018Article
- Modified insulin-like growth factor 1 containing collagen-binding domain for nerve regeneration.Neural regeneration research · 2018Article
- High-speed atomic force microscopy reveals strongly polarized movement of clostridial collagenase along collagen fibrils.Scientific reports · 2016Article
- Structures of three polycystic kidney disease-like domains from Clostridium histolyticum collagenases ColG and ColH.Acta crystallographica. Section D, Biological crystallography · 2015Article
- Mimicking the hierarchical functions of dentin collagen cross-links with plant derived phenols and phenolic acids.Langmuir : the ACS journal of surfaces and colloids · 2014Article
- Proteomic protease specificity profiling of clostridial collagenases reveals their intrinsic nature as dedicated degraders of collagen.Journal of proteomics · 2014Article
- Interstitial collagen catabolism.The Journal of biological chemistry · 2013Review
- Structural comparison of ColH and ColG collagen-binding domains from Clostridium histolyticum.Journal of bacteriology · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 4 institutions in 2 countries.
Funding
Abstract
Clostridium histolyticum collagenase causes extensive degradation of collagen in connective tissue that results in gas gangrene. The C-terminal collagen-binding domain (CBD) of these enzymes is the minimal segment required to bind to a collagen fibril. CBD binds unidirectionally to the undertwisted C-terminus of triple helical collagen. Here, we examine whether CBD could also target undertwisted regions even in the middle of the triple helix. Collageneous peptides with an additional undertwisted region were synthesized by introducing a Gly → Ala substitution [(POG)(x) POA(POG)(y)]₃, where x + y = 9 and x > 3). ¹H-¹⁵N heteronuclear single quantum coherence nuclear magnetic resonance (HSQC NMR) titration studies with ¹⁵N-labeled CBD demonstrated that the minicollagen binds to a 10 Å wide 25 Å long cleft. Six collagenous peptides each labeled with a nitroxide radical were then titrated with ¹⁵N-labeled CBD. CBD binds to either the Gly → Ala substitution site or to the C-terminus of each minicollagen. Small-angle X-ray scattering measurements revealed that CBD prefers to bind the Gly → Ala site to the C-terminus. The HSQC NMR spectra of ¹⁵N-labeled minicollagen and minicollagen with undertwisted regions were unaffected by the titration of unlabeled CBD. The results imply that CBD binds to the undertwisted region of the minicollagen but does not actively unwind the triple helix.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.