ArticleJournal of assisted reproduction and genetics2012
Optimizing cryoprotectant perfusion conditions for intact ovary: a bovine model.
Article in Journal of assisted reproduction and genetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed.
- Whole Ovary Cryopreservation and Transplantation: A Systematic Review of Challenges and Research Developments in Animal Experiments and Humans.Journal of clinical medicine · 2020Review
- Dimethyl Sulfoxide is Less Effective in Immersing Cryopreserved Large Pieces of Tissue: A Rabbit Hind-Limb Model.Medical science monitor : international medical journal of experimental and clinical research · 2019Article
- Successful fertility following optimized perfusion and cryopreservation of whole ovary and allotransplantation in a premature ovarian insufficiency rat model.Journal of ovarian research · 2018Article
- Complete protection against cryodamage of cryopreserved whole bovine and human ovaries using DMSO as a cryoprotectant.Journal of assisted reproduction and genetics · 2017Article
- Successful cryopreservation of whole sheep ovary by using DMSO-free cryoprotectant.Journal of assisted reproduction and genetics · 2015Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe aim of this study was to detect the effects of different perfusion pressure and different length of perfusion period on whole ovarian cryopreservation
methodsBovine whole ovaries were vitrified-warmed. The ovaries were divided into the experimental groups according to different perfusion pressure and different length of perfusion period. Follicular viability was assessed using the trypan blue test; the percentage of morphologically normal primordial follicles and the 17-β estradiol level in the culture supernatants were measured.
resultsWhen perfusion pressure was 100 mmHg, and the length of perfusion period was 40 min, the viability of ovarian tissues in bovine whole ovarian cryopreservation were higher than other protocols.
conclusionProtocol IIb (the perfusion pressure was 100 mmHg, and the length of perfusion period was 40 min) was appropriate for bovine whole ovarian cryopreservation.
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Registered trials
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