ArticleDifferentiation; research in biological diversity2012
Visualization and quantification of mouse prostate development by in situ hybridization.
Article in Differentiation; research in biological diversity, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 33 citations in OpenAlex.
- Article
- Prostate organogenesis.Development (Cambridge, England) · 2022Article
- Cholesterol Contributes to Male Sex Differentiation Through Its Developmental Role in Androgen Synthesis and Hedgehog Signaling.Endocrinology · 2021Review
- RNA-binding protein DDX3 mediates posttranscriptional regulation of androgen receptor: A mechanism of castration resistance.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- RUNX1 marks a luminal castration-resistant lineage established at the onset of prostate development.eLife · 2020Article
- GLI3 resides at the intersection of hedgehog and androgen action to promote male sex differentiation.PLoS genetics · 2020Article
- Article
- Sox9 in mouse urogenital sinus epithelium mediates elongation of prostatic buds and expression of genes involved in epithelial cell migration.Gene expression patterns : GEP · 2019Article
- A temporal and spatial map of axons in developing mouse prostate.Histochemistry and cell biology · 2019Article
- Edar is a downstream target of beta-catenin and drives collagen accumulation in the mouse prostate.Biology open · 2019Article
- Epithelial DNA methyltransferase-1 regulates cell survival, growth and maturation in developing prostatic buds.Developmental biology · 2019Article
- Prostate Organogenesis.Cold Spring Harbor perspectives in medicine · 2018Review
- Species and Sex Differences in the Morphogenic Response of Primary Rodent Neurons to 3,3'-Dichlorobiphenyl (PCB 11).Toxics · 2017Article
- In vivo and in vitro sex differences in the dendritic morphology of developing murine hippocampal and cortical neurons.Scientific reports · 2017Article
- Prostate organogenesis: tissue induction, hormonal regulation and cell type specification.Development (Cambridge, England) · 2017Review
- Histone acetylation regulates prostate ductal morphogenesis through a bone morphogenetic protein-dependent mechanism.Developmental dynamics : an official publication of the American Association of Anatomists · 2015Article
- An illustrated anatomical ontology of the developing mouse lower urogenital tract.Development (Cambridge, England) · 2015Article
- Article
- Androgen receptor DNA methylation regulates the timing and androgen sensitivity of mouse prostate ductal development.Developmental biology · 2014Article
- DNA methylation of E-cadherin is a priming mechanism for prostate development.Developmental biology · 2014Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The purpose of this study was to validate a combined in situ hybridization (ISH)/immunohistochemistry (IHC) staining method for visualizing and quantifying mouse prostatic buds. To refine animal usage in prostate development studies, we also determined whether a comparable number of prostatic buds were formed in male and female mouse urogenital sinus (UGS) explants grown in vitro in the presence of androgen. We used IHC to label UGS epithelium and ISH to label prostatic buds with one of three different prostatic bud marking riboprobes: a previously identified prostatic bud marker, NK-3 transcription factor, locus 1 (Nkx3-1), and two newly identified prostatic bud markers, wingless-related MMTV integration site 10b (Wnt10b) and ectodysplasin-A receptor (Edar). We calculated total buds formed per UGS and the proportion marked by each mRNA after male UGS development in vivo and male and female UGS development in vitro. Nkx3-1 was first to mark the prostate field during UGS development in vivo but all three mRNAs marked prostatic buds during later developmental stages. The mRNAs localized to different domains: Nkx3-1 was present along about half the prostatic bud length while Edar and Wnt10b were restricted to distal bud tips. None of the mRNAs marked all buds formed in vitro and the proportion marked was developmental stage- and gender-dependent. Nkx3-1 marked the highest proportion of prostatic buds during in vitro UGS development. Together, our results reveal that ISH staining of mouse UGS can be used to quantify prostatic bud number, Nkx3-1 is currently the best suited riboprobe for this method, and female UGSs cannot be used interchangeably with male UGSs when conducting prostate development studies in vitro. We also found that Nkx3-1, Edar, and Wnt10b mark different prostatic bud regions and are likely to be useful in future studies of regional differences in prostatic bud gene expression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.