Trial reportParasites & vectors2012
Characterization and evaluation of a Sarcoptes scabiei allergen as a candidate vaccine.
Trial report in Parasites & vectors, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 40 citations in OpenAlex.
- Antiparasitic efficacy of geraniol from Apiaceae family in scabies treatment.Scientific reports · 2025Article
- Scabies.Nature reviews. Disease primers · 2024Review
- Mange in Rabbits: An Ectoparasitic Disease with a Zoonotic Potential.Veterinary medicine international · 2022Review
- An Antibody Persistent and Protective Two rSsCLP-Based Subunit Cocktail Vaccine againstVaccines · 2020Article
- Tropomyosin: An Excretory/Secretory Protein fromVaccines · 2020Article
- Characterization of mitochondrial COX-1 gene of Sarcoptes scabiei from rabbits in East Java, Indonesia.Journal of advanced veterinary and animal research · 2019Article
- Sequence analysis of the cytochrome c oxidase subunit 1 gene ofVeterinary world · 2019Article
- A chitinase-like protein from Sarcoptes scabiei as a candidate anti-mite vaccine that contributes to immune protection in rabbits.Parasites & vectors · 2018Article
- Serodiagnostic Potential of Alpha-Enolase FromFrontiers in microbiology · 2018Article
- Characterization ofThe American journal of tropical medicine and hygiene · 2017Article
- Expression and characterisation of a Sarcoptes scabiei protein tyrosine kinase as a potential antigen for scabies diagnosis.Scientific reports · 2017Article
- A review of Sarcoptes scabiei: past, present and future.Parasites & vectors · 2017Review
- Article
- Characterisation of tropomyosin and paramyosin as vaccine candidate molecules for the poultry red mite, Dermanyssus gallinae.Parasites & vectors · 2016Article
- Vaccination of rabbits with immunodominant antigens from Sarcoptes scabiei induced high levels of humoral responses and pro-inflammatory cytokines but confers limited protection.Parasites & vectors · 2016Article
- The emergence of sarcoptic mange in Australian wildlife: an unresolved debate.Parasites & vectors · 2016Review
- Exploration ofIranian journal of parasitologyArticle
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSarcoptic mange caused by the mite Sarcoptes scabiei is a worldwide disease affecting both humans and animals. Here we report the molecular characterization and evaluation of a recombinant S. scabiei tropomyosin (SsTm) protein in a vaccination trial in rabbits.
methodsThe full-length cDNA was cloned in a bacterial pET vector, and the recombinant protein was expressed in BL21 (DE3) cells and purified. Using specific rabbit antiserum, tropomyosin was localized immunohistochemically in mite tissue sections. Vaccination trials with the recombiant SsTm was carried out in New Zealand rabbits.
resultsThe full-length open reading frame (ORF) of the 852 bp cloned gene from S. scabiei encodes a 32.9 kDa protein. The amino acid sequence showed 98.94%, 97.89% and 98.59% homology to Dermatophagoides farina and Dermatophagoides pteronyssinus group 10 allergens and Psoroptes ovis tropomyosin, respectively. Tropomyosin was localized immunohistochemically in mite tissue sections mainly in the mouthparts, legs and integument of the epidermis. The predicted cross-reactivity of SsTm indicated that it is an allergenic protein. While vaccination with the recombiant SsTm resulted in high levels of specific IgG (P < 0.01), a low IgE antibody response and no significant protection against S. scabiei challenge were observed. After challenge, specific IgG levels remained significantly higher than the control (P < 0.01), while changes of total IgE levels were not significant (P > 0.05). However, the lesion areas in the vaccination group decreased at the end of the experiment compared with controls.
conclusionsAlthough vaccination with recombinant SsTm did not efficiently control sarcoptic mange in rabbits, the immunogenic properties of tropomyosin suggest it may be developed as a vaccine with alternative adjuvants or delivery methods.
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