ArticleMolecular microbiology2012
Deletion of mitochondrial associated ubiquitin fold modifier protein Ufm1 in Leishmania donovani results in loss of β-oxidation of fatty acids and blocks cell division in the amastigote stage.
Article in Molecular microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 47 citations in OpenAlex.
- Next-Generation Leishmanization: Revisiting Molecular Targets for Selecting Genetically Engineered Live-AttenuatedMicroorganisms · 2023Review
- Genetic model of UBA5 deficiency highlights the involvement of both peripheral and central nervous systems and identifies widespread mitochondrial abnormalities.Brain communications · 2023Article
- Modification of ERα by UFM1 Increases Its Stability and Transactivity for Breast Cancer Development.Molecules and cells · 2022Article
- Three types ofFrontiers in cellular and infection microbiology · 2022Article
- A description of novel variants and review of phenotypic spectrum inCold Spring Harbor molecular case studies · 2021Article
- UFL1 regulates milk protein and fat synthesis-related gene expression of bovine mammary epithelial cells probably via the mTOR signaling pathway.In vitro cellular & developmental biology. Animal · 2021Article
- DDX3 DEAD-box RNA helicase (Hel67) gene disruption impairs infectivity of Leishmania donovani and induces protective immunity against visceral leishmaniasis.Scientific reports · 2020Article
- TheGenes · 2020Article
- Ubiquitin-Like Modifiers: Emerging Regulators of Protozoan Parasites.Biomolecules · 2020Review
- Leishmania differentiation requires ubiquitin conjugation mediated by a UBC2-UEV1 E2 complex.PLoS pathogens · 2020Article
- Article
- A new gene inventory of the ubiquitin and ubiquitin-like conjugation pathways in Giardia intestinalis.Memorias do Instituto Oswaldo Cruz · 2020Article
- UFM1 suppresses invasive activities of gastric cancer cells by attenuating the expres7sion of PDK1 through PI3K/AKT signaling.Journal of experimental & clinical cancer research : CR · 2019Article
- Ufm1-Specific Ligase Ufl1 Regulates Endoplasmic Reticulum Homeostasis and Protects Against Heart Failure.Circulation. Heart failure · 2018Article
- A Leishmania-specific gene upregulated at the amastigote stage is crucial for parasite survival.Parasitology research · 2018Article
- Article
- UFMylation: A Unique & Fashionable Modification for Life.Genomics, proteomics & bioinformatics · 2016Review
- Standardized methods to generate mock (spiked) clinical specimens by spiking blood or plasma with cultured pathogens.Journal of applied microbiology · 2016Article
- Leishmania carbon metabolism in the macrophage phagolysosome- feast or famine?F1000Research · 2015Review
- The ufm1 cascade.Cells · 2014Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Recently, we described the existence of the ubiquitin fold modifier 1 (Ufm1) and its conjugation pathway in Leishmania donovani. We demonstrated the conjugation of Ufm1 to proteins such as mitochondrial trifunctional protein (MTP) that catalyses β-oxidation of fatty acids in L. donovani. To elucidate the biological roles of the Ufm1-mediated modifications, we made an L. donovani Ufm1 null mutant (Ufm1(-/-)). Loss of Ufm1 and consequently absence of Ufm1 conjugation with MTP resulted in diminished acetyl-CoA, the end-product of the β-oxidation in the Ufm1(-/-) amastigote stage. The Ufm1(-/-) mutants showed reduced survival in the amastigote stage in vitro and ex vivo in human macrophages. This survival was restored by re-expression of wild-type Ufm1 with concomitant induction of acetyl-CoA but not by re-expressing the non-conjugatable Ufm1, indicating the essential nature of Ufm1 conjugation and β-oxidation. Both cell cycle analysis and ultrastructural studies of Ufm1(-/-) parasites confirmed the role of Ufm1 in amastigote growth. The defect in vitro growth of amastigotes in human macrophages was further substantiated by reduced survival. Therefore, these studies suggest the importance of Ufm1 in Leishmania pathogenesis with larger impact on other organisms and further provide an opportunity to test Ufm1(-/-) parasites as drug and vaccine targets.
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