Evidence map›Paper›PMID 22897044›Full record

ArticleJournal of medical entomology2012

Identification of endosymbionts in ticks by broad-range polymerase chain reaction and electrospray ionization mass spectrometry.

Megan A Rounds, Christopher D Crowder, Heather E Matthews, Curtis A Philipson, Glen A Scoles, David J Ecker, Steven E Schutzer, Mark W Eshoo

Open access · bronzeAbstract read
In one paragraph

Article in Journal of medical entomology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
5.8field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 42 citations in OpenAlex.

  1. Xenodiagnosis to detect Borrelia burgdorferi infection: a first-in-human study.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2014
    Trial
  2. Article
  3. Recently EvolvedFrontiers in cellular and infection microbiology · 2022
    Article
  4. Article
  5. Review
  6. Ticks and Tularemia: Do We Know What We Don't Know?Frontiers in cellular and infection microbiology · 2019
    Review
  7. Article
  8. Article
  9. Article
  10. Review
  11. Review
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Review
  18. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Megan A RoundsIbis Biosciences, Inc., an Abbott Company, Carlsbad, CA 92008, USA.
Christopher D Crowder
Heather E Matthews
Curtis A Philipson
Glen A Scoles
David J Ecker
Steven E Schutzer
Mark W Eshoo
Ionis Pharmaceuticals (United States) · USNational Animal Disease Center · USRutgers, The State University of New Jersey · US

Funding

Broad Detection System for Tick-Borne PathogensR44AI077156 · NIAID · BIOSCIENCE DEVELOPMENT, INC. · PI SCHUTZER, STEVEN E · 2009 to 2011
$3.0M
Broad Detection System for Tick-Borne PathogensR43AI077156 · NIAID · IBIS BIOSCIENCES, INC. · PI ESHOO, MARK W · 2008 to 2008
$96k
NIAID NIH HHS 2R44AI077156-02NIAID NIH HHS R43 AI077156NIAID NIH HHS R44 AI077156
6 · The paper itself

Abstract

Many organisms, such as insects, filarial nematodes, and ticks, contain heritable bacterial endosymbionts that are often closely related to transmissible tickborne pathogens. These intracellular bacteria are sometimes unique to the host species, presumably due to isolation and genetic drift. We used a polymerase chain reaction/electrospray ionization-mass spectrometry assay designed to detect a wide range of vectorborne microorganisms to characterize endosymbiont genetic signatures from Amblyomma americanum (L.), Amblyomma maculatum Koch, Dermacentor andersoni Stiles, Dermacentor occidentalis Marx, Dermacentor variabilis (Say), Ixodes scapularis Say, Ixodes pacificus Cooley & Kohls, Ixodes ricinus (L.), and Rhipicephalus sanguineus (Latreille) ticks collected at various sites and of different stages and both sexes. The assay combines the abilities to simultaneously detect pathogens and closely related endosymbionts and to identify tick species via characterization of their respective unique endosymbionts in a single test.

Indexed as

SymbiosisAnimalsIxodidaeLarvaNymphOvumPolymerase Chain ReactionRickettsiaSpecies SpecificitySpectrometry, Mass, Electrospray Ionization

Identifiers

PMID22897044
PMCPMC3535486
OpenAlexW2094337584

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.