ArticleJournal of lipid research2012
A human phospholipid phosphatase activated by a transmembrane control module.
Article in Journal of lipid research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
- From Plankton to Primates: How VSP Sequence Diversity Shapes Voltage Sensing.International journal of molecular sciences · 2025Article
- Myosin-1C augments endothelial secretion of von Willebrand factor by linking contractile actomyosin machinery to the plasma membrane.Blood advances · 2024Article
- Hydrophobic residues in S1 modulate enzymatic function and voltage sensing in voltage-sensing phosphatase.The Journal of general physiology · 2024Article
- Predicting the Structural Impact of Human Alternative Splicing.bioRxiv : the preprint server for biology · 2023Article
- Role of K364 next to the active site cysteine in voltage-dependent phosphatase activity of Ci-VSP.Biophysical journal · 2023Article
- Exome sequencing reveals variants in known and novel candidate genes for severe sperm motility disorders.Human reproduction (Oxford, England) · 2021Article
- Discovery and characterization of HeLife · 2021Article
- Modulation of KFrontiers in pharmacology · 2020Article
- A126 in the active site and TI167/168 in the TI loop are essential determinants of the substrate specificity of PTEN.Cellular and molecular life sciences : CMLS · 2018Article
- OverexpressingOncotarget · 2018Article
- KChannels (Austin, Tex.) · 2018Article
- Domain-to-domain coupling in voltage-sensing phosphatase.Biophysics and physicobiology · 2017Review
- Characterization of the Functional Domains of a Mammalian Voltage-Sensitive Phosphatase.Biophysical journal · 2015Article
- Discovery and functional characterization of a neomorphic PTEN mutation.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- A method to control phosphoinositides and to analyze PTEN function in living cells using voltage sensitive phosphatases.Frontiers in pharmacology · 2015Article
- Voltage sensitive phosphatases: emerging kinship to protein tyrosine phosphatases from structure-function research.Frontiers in pharmacology · 2015Review
- Sensing charges of the Ciona intestinalis voltage-sensing phosphatase.The Journal of general physiology · 2013Article
- Voltage-Controlled Enzymes: The New JanusBifrons.Frontiers in pharmacology · 2012Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In voltage-sensitive phosphatases (VSPs), a transmembrane voltage sensor domain (VSD) controls an intracellular phosphoinositide phosphatase domain, thereby enabling immediate initiation of intracellular signals by membrane depolarization. The existence of such a mechanism in mammals has remained elusive, despite the presence of VSP-homologous proteins in mammalian cells, in particular in sperm precursor cells. Here we demonstrate activation of a human VSP (hVSP1/TPIP) by an intramolecular switch. By engineering a chimeric hVSP1 with enhanced plasma membrane targeting containing the VSD of a prototypic invertebrate VSP, we show that hVSP1 is a phosphoinositide-5-phosphatase whose predominant substrate is PI(4,5)P(2). In the chimera, enzymatic activity is controlled by membrane potential via hVSP1's endogenous phosphoinositide binding motif. These findings suggest that the endogenous VSD of hVSP1 is a control module that initiates signaling through the phosphatase domain and indicate a role for VSP-mediated phosphoinositide signaling in mammals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.