ReviewJournal of the Royal Society, Interface2012
Recent advances in mapping environmental microbial metabolisms through 13C isotopic fingerprints.
Review in Journal of the Royal Society, Interface, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Microbial contribution to metabolic niche formation varies across the respiratory tract.Cell host & microbe · 2025Article
- An isotopic labeling approach linking natural products with biosynthetic gene clusters.Nature chemical biology · 2022Article
- Model metabolic strategy for heterotrophic bacteria in the cold ocean based onProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Bacterial Substrate Transformation Tracked by Stable-Isotope-Guided NMR Metabolomics: Application in a Natural Aquatic Microbial Community.Metabolites · 2017Article
- Decoding how a soil bacterium extracts building blocks and metabolic energy from ligninolysis provides road map for lignin valorization.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Rapid Prediction of Bacterial Heterotrophic Fluxomics Using Machine Learning and Constraint Programming.PLoS computational biology · 2016Article
- Two pathways for glutamate biosynthesis in the syntrophic bacterium Syntrophus aciditrophicus.Applied and environmental microbiology · 2015Article
- The return of metabolism: biochemistry and physiology of the pentose phosphate pathway.Biological reviews of the Cambridge Philosophical Society · 2015Review
- Large-Scale 13C flux profiling reveals conservation of the Entner-Doudoroff pathway as a glycolytic strategy among marine bacteria that use glucose.Applied and environmental microbiology · 2015Article
- Characterization of the pivotal carbon metabolism of Streptococcus suis serotype 2 under ex vivo and chemically defined in vitro conditions by isotopologue profiling.The Journal of biological chemistry · 2015Article
- Recent highlights in biosynthesis research using stable isotopes.Beilstein journal of organic chemistry · 2015Review
- Article
- Elucidation of intrinsic biosynthesis yields using 13C-based metabolism analysis.Microbial cell factories · 2014Review
- The use of functional genomics in conjunction with metabolomics for Mycobacterium tuberculosis research.Disease markers · 2014Review
- Experimental evidence and isotopomer analysis of mixotrophic glucose metabolism in the marine diatom Phaeodactylum tricornutum.Microbial cell factories · 2013Article
- Metabolic flux analysis of Cyanothece sp. ATCC 51142 under mixotrophic conditions.Photosynthesis research · 2013Article
- Development of Synechocystis sp. PCC 6803 as a phototrophic cell factory.Marine drugs · 2013Review
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
After feeding microbes with a defined (13)C substrate, unique isotopic patterns (isotopic fingerprints) can be formed in their metabolic products. Such labelling information not only can provide novel insights into functional pathways but also can determine absolute carbon fluxes through the metabolic network via metabolic modelling approaches. This technique has been used for finding pathways that may have been mis-annotated in the past, elucidating new enzyme functions, and investigating cell metabolisms in microbial communities. In this review paper, we summarize the applications of (13)C approaches to analyse novel cell metabolisms for the past 3 years. The isotopic fingerprints (defined as unique isotopomers useful for pathway identifications) have revealed the operations of the Entner-Doudoroff pathway, the reverse tricarboxylic acid cycle, new enzymes for biosynthesis of central metabolites, diverse respiration routes in phototrophic metabolism, co-metabolism of carbon nutrients and novel CO(2) fixation pathways. This review also discusses new isotopic methods to map carbon fluxes in global metabolisms, as well as potential factors influencing the metabolic flux quantification (e.g. metabolite channelling, the isotopic purity of (13)C substrates and the isotopic effect). Although (13)C labelling is not applicable to all biological systems (e.g. microbial communities), recent studies have shown that this method has a significant value in functional characterization of poorly understood micro-organisms, including species relevant for biotechnology and human health.
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