ArticleAmerican journal of physiology. Renal physiology2012
NADPH oxidase 4 mediates flow-induced superoxide production in thick ascending limbs.
Article in American journal of physiology. Renal physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 30 citations in OpenAlex.
- Luminal flow in the connecting tubule induces afferent arteriole vasodilation.Clinical and experimental nephrology · 2025Article
- Luminal Flow in the Connecting Tubule induces Afferent Arteriole Vasodilation.bioRxiv : the preprint server for biology · 2024Article
- Hydrogen peroxide (HScientific reports · 2021Article
- Stretch-Induced Increases in Intracellular Ca Stimulate Thick Ascending Limb OHypertension (Dallas, Tex. : 1979) · 2020Article
- Angiotensin II-induced superoxide and decreased glutathione in proximal tubules: effect of dietary fructose.American journal of physiology. Renal physiology · 2020Article
- Involvement of Vanin-1 in Ameliorating Effect of Oxidative Renal Tubular Injury in Dahl-Salt Sensitive Rats.International journal of molecular sciences · 2019Article
- Effects of reactive oxygen species on renal tubular transport.American journal of physiology. Renal physiology · 2019Review
- Thick Ascending Limb Sodium Transport in the Pathogenesis of Hypertension.Physiological reviews · 2019Review
- NADPH oxidase 4-derived superoxide mediates flow-stimulated NKCC2 activity in thick ascending limbs.American journal of physiology. Renal physiology · 2018Article
- Effects of Reactive Oxygen Species on Tubular Transport along the Nephron.Antioxidants (Basel, Switzerland) · 2017Review
- Diabetes and Kidney Disease: Role of Oxidative Stress.Antioxidants & redox signaling · 2016Review
- Role of Nox4 and p67phox subunit of Nox2 in ROS production in response to increased tubular flow in the mTAL of Dahl salt-sensitive rats.American journal of physiology. Renal physiology · 2016Article
- Paracellular epithelial sodium transport maximizes energy efficiency in the kidney.The Journal of clinical investigation · 2016Article
- Luminal flow induces NADPH oxidase 4 translocation to the nuclei of thick ascending limbs.Physiological reports · 2016Article
- Angiotensin II stimulates superoxide production by nitric oxide synthase in thick ascending limbs.Physiological reports · 2016Article
- Evidence of the Importance of Nox4 in Production of Hypertension in Dahl Salt-Sensitive Rats.Hypertension (Dallas, Tex. : 1979) · 2016Article
- Effects of p67phox on the mitochondrial oxidative state in the kidney of Dahl salt-sensitive rats: optical fluorescence 3-D cryoimaging.American journal of physiology. Renal physiology · 2015Article
- Endogenous flow-induced nitric oxide reduces superoxide-stimulated Na/H exchange activity via PKG in thick ascending limbs.American journal of physiology. Renal physiology · 2015Article
- Reactive oxygen species as important determinants of medullary flow, sodium excretion, and hypertension.American journal of physiology. Renal physiology · 2015Review
- A radical approach to balancing the tides of tubular flow.American journal of physiology. Renal physiology · 2014Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
We previously showed that luminal flow stimulates thick ascending limb (TAL) superoxide (O(2)(-)) production by stretching epithelial cells and increasing NaCl transport, and reported that the major source of flow-induced O(2)(-) is NADPH oxidase (Nox). However, the specific Nox isoform involved is unknown. Of the three isoforms expressed in the kidney-Nox1, Nox2, and Nox4-we hypothesized that Nox4 is responsible for flow-induced O(2)(-) production in TALs. Measurable flow-induced O(2)(-) production at physiological flow rates of 0, 5, 10, and 20 nl/min was 5 ± 1, 9 ± 2, 36 ± 6, and 66 ± 8 AU/s, respectively. RT-PCR detected mRNA for all three Nox isoforms in the TAL. The order of RNA abundance was Nox2 > Nox4 >>> Nox1. Since all three isoforms are expressed in TALs and pharmacological inhibitors are not selective, we used rats transduced with siRNA and knockout mice. Nox4 siRNA knocked down Nox4 mRNA expression by 63 ± 7% but did not reduce Nox1 or Nox2 mRNA. Flow-induced O(2)(-) was 18 ± 9 AU/s in TALs transduced with Nox4 siRNA compared with 77 ± 9 AU/s in tubules transduced with scrambled siRNA. Flow-induced O(2)(-) was 81 ± 5 AU/s in Nox2 knockout mice compared with 83 ± 13 AU/s in wild-type mice. In TALs transduced with Nox1 siRNA, flow-induced O(2)(-) was 82 ± 7 AU/s. We conclude that Nox4 mediates flow-induced O(2)(-) production in TALs.
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