Evidence map›Paper›PMID 22895712›Full record

ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2012

Regulation of postnatal forebrain amoeboid microglial cell proliferation and development by the transcription factor Runx1.

Morena Zusso, Laurent Methot, Rita Lo, Andrew D Greenhalgh, Samuel David, Stefano Stifani

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.

0numbers the graph read from it
0cells of the map it votes in
86citing papers in PubMed
6.0field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

86 citing papers in PubMed, 158 citations in OpenAlex.

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26 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 3 countries.

Morena ZussoMontreal Neurological Institute, McGill University, Montreal, Quebec, H3A 2B4 Canada.
Laurent Methot
Rita Lo
Andrew D Greenhalgh
Samuel David
Stefano Stifani
McGill University · CAThe Neurological Institute · US

Funding

Canadian Institutes of Health Research MOP-14828Canadian Institutes of Health Research MOP-42479Canadian Institutes of Health Research MOP-84577
6 · The paper itself

Abstract

Microglia are the immune cells of the nervous system, where they act as resident macrophages during inflammatory events underlying many neuropathological conditions. Microglia derive from primitive myeloid precursors that colonize the nervous system during embryonic development. In the postnatal brain, microglia are initially mitotic, rounded in shape (amoeboid), and phagocytically active. As brain development proceeds, they gradually undergo a transition to a surveillant nonphagocytic state characterized by a highly branched (ramified) morphology. This ramification process is almost recapitulated in reverse during the process of microglia activation in the adult brain, when surveillant microglia undergo a ramified-to-amoeboid morphological transformation and become phagocytic in response to injury or disease. Little is known about the mechanisms controlling amoeboid microglial cell proliferation, activation, and ramification during brain development, despite the critical role of these processes in the establishment of the adult microglia pool and their relevance to microglia activation in the adult brain. Here we show that the mouse transcription factor Runx1, a key regulator of myeloid cell proliferation and differentiation, is expressed in forebrain amoeboid microglia during the first two postnatal weeks. Runx1 expression is then downregulated in ramified microglia. Runx1 inhibits mouse amoeboid microglia proliferation and promotes progression to the ramified state. We show further that Runx1 expression is upregulated in microglia following nerve injury in the adult mouse nervous system. These findings provide insight into the regulation of postnatal microglia activation and maturation to the ramified state and have implications for microglia biology in the developing and injured brain.

Indexed as

Cell ProliferationAnimalsAnimals, NewbornAntigens, DifferentiationBromodeoxyuridineCalcium-Binding ProteinsCD11b AntigenCell Line, TransformedCells, CulturedChromatin ImmunoprecipitationCore Binding Factor Alpha 2 SubunitCyclin-Dependent Kinase Inhibitor p21Embryo, MammalianFemaleGene Expression Regulation, DevelopmentalGreen Fluorescent ProteinsAif1 protein, mouseAntigens, DifferentiationBromodeoxyuridineCalcium-Binding ProteinsCD11b AntigenCore Binding Factor Alpha 2 SubunitCyclin-Dependent Kinase Inhibitor p21Green Fluorescent ProteinsIntermediate Filament ProteinsKi-67 AntigenMicrofilament Proteinsmonocyte-macrophage differentiation antigenNerve Tissue ProteinsNES protein, humanNes protein, mouseNestinNitric Oxide Synthase Type IIPhosphatidylethanolaminesRunx1 protein, mousetransfectACE

Identifiers

PMID22895712
PMCPMC6621177
OpenAlexW2142187403

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.