ReviewFunctional & integrative genomics2012
Chromosomes in the flow to simplify genome analysis.
Review in Functional & integrative genomics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
52 citing papers in PubMed, 115 citations in OpenAlex.
- Cytogenomic profiling ofJournal of ginseng research · 2026Article
- Genetic diversity of the Y-chromosome in domestic animals: from evolutionary insights to functional implications.Molecular biology reports · 2025Review
- Whole-genome sequencing in medicinal plants: current progress and prospect.Science China. Life sciences · 2024Review
- Barley MLA3 recognizes the host-specificity effector Pwl2 from Magnaporthe oryzae.The Plant cell · 2024Article
- An effective strategy for assembling the sex-limited chromosome.GigaScience · 2024Article
- Y chromosome sequence and epigenomic reconstruction across human populations.Communications biology · 2023Article
- GALA: a computational framework for de novo chromosome-by-chromosome assembly with long reads.Nature communications · 2023Article
- Comparative genomic analysis of 5MFrontiers in plant science · 2023Article
- Flow Cytometric Analysis and Sorting of Plant Chromosomes.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Isolation and Sequencing of Chromosome Arm 7RS of Rye,International journal of molecular sciences · 2022Article
- Modest increase of KIF11 expression exposes fragilities in the mitotic spindle, causing chromosomal instability.Journal of cell science · 2022Article
- A lineage-specific Exo70 is required for receptor kinase-mediated immunity in barley.Science advances · 2022Article
- Flow karyotyping of wheat-Frontiers in plant science · 2022Article
- Chromosome analysis and sorting.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2021Review
- A membrane-bound ankyrin repeat protein confers race-specific leaf rust disease resistance in wheat.Nature communications · 2021Article
- Bridging the Gap between Vertebrate Cytogenetics and Genomics with Single-Chromosome Sequencing (ChromSeq).Genes · 2021Review
- Proteome Analysis of Condensed Barley Mitotic Chromosomes.Frontiers in plant science · 2021Article
- Breeding and Economic Aspects of Cytogenetic Screening Studies of Pigs Qualified for Reproduction.Animals : an open access journal from MDPI · 2020Review
- Comparative analysis of chromosome 2A molecular organization in diploid and hexaploid wheat.Molecular biology reports · 2020Article
- Plant Chromosome-Specific Probes by Microdissection of a Single Chromosome: Is That a Reality?Frontiers in plant science · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclear genomes of human, animals, and plants are organized into subunits called chromosomes. When isolated into aqueous suspension, mitotic chromosomes can be classified using flow cytometry according to light scatter and fluorescence parameters. Chromosomes of interest can be purified by flow sorting if they can be resolved from other chromosomes in a karyotype. The analysis and sorting are carried out at rates of 10(2)-10(4) chromosomes per second, and for complex genomes such as wheat the flow sorting technology has been ground-breaking in reducing genome complexity for genome sequencing. The high sample rate provides an attractive approach for karyotype analysis (flow karyotyping) and the purification of chromosomes in large numbers. In characterizing the chromosome complement of an organism, the high number that can be studied using flow cytometry allows for a statistically accurate analysis. Chromosome sorting plays a particularly important role in the analysis of nuclear genome structure and the analysis of particular and aberrant chromosomes. Other attractive but not well-explored features include the analysis of chromosomal proteins, chromosome ultrastructure, and high-resolution mapping using FISH. Recent results demonstrate that chromosome flow sorting can be coupled seamlessly with DNA array and next-generation sequencing technologies for high-throughput analyses. The main advantages are targeting the analysis to a genome region of interest and a significant reduction in sample complexity. As flow sorters can also sort single copies of chromosomes, shotgun sequencing DNA amplified from them enables the production of haplotype-resolved genome sequences. This review explains the principles of flow cytometric chromosome analysis and sorting (flow cytogenetics), discusses the major uses of this technology in genome analysis, and outlines future directions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.