ArticleLab on a chip2012
Real-time full-spectral imaging and affinity measurements from 50 microfluidic channels using nanohole surface plasmon resonance.
Article in Lab on a chip, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- SPR Sensing: From Biomolecular Interactions to Cell-Based Analysis.Biosensors · 2026Review
- A Comparison of Cost-Effective Sensors for a Fluorescence-Based Detection System Used in Biodiagnostic Devices.Biosensors · 2026Article
- Article
- Binding Kinetics and Epitope Binning Using Surface Plasmon Resonance.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Emerging Designs and Applications for Biomembrane Biosensors.Annual review of analytical chemistry (Palo Alto, Calif.) · 2024Review
- Fibre-Optic Surface Plasmon Resonance Biosensor for Monoclonal Antibody Titer Quantification.Biosensors · 2021Article
- Microfluidics-Based Plasmonic Biosensing System Based on Patterned Plasmonic Nanostructure Arrays.Micromachines · 2021Review
- Pressure Sensitive Adhesive Tape: A Versatile Material Platform for Optical Sensors.Sensors (Basel, Switzerland) · 2020Review
- Optical Interrogation Techniques for Nanophotonic Biochemical Sensors.Sensors (Basel, Switzerland) · 2019Review
- Recent Advances in Surface Plasmon Resonance Imaging Sensors.Sensors (Basel, Switzerland) · 2019Review
- Spectral Imaging Analysis for Ultrasensitive Biomolecular Detection Using Gold-Capped Nanowire Arrays.Sensors (Basel, Switzerland) · 2018Article
- Superior LSPR substrates based on electromagnetic decoupling for on-a-chip high-throughput label-free biosensing.Light, science & applications · 2017Article
- Probing the Interaction of Dielectric Nanoparticles with Supported Lipid Membrane Coatings on Nanoplasmonic Arrays.Sensors (Basel, Switzerland) · 2017Article
- Study of flow rate induced measurement error in flow-through nano-hole plasmonic sensor.Biomicrofluidics · 2015Article
- Localized Surface Plasmon Resonance Biosensing: Current Challenges and Approaches.Sensors (Basel, Switzerland) · 2015Review
- Various on-chip sensors with microfluidics for biological applications.Sensors (Basel, Switzerland) · 2014Review
- Advances in plasmonic technologies for point of care applications.Chemical reviews · 2014Review
- Transmissive Nanohole Arrays for Massively-Parallel Optical Biosensing.ACS photonics · 2014Article
- Micro total analysis systems: fundamental advances and biological applications.Analytical chemistry · 2014Review
- Promises and Challenges of Nanoplasmonic Devices for Refractometric Biosensing.Nanophotonics (Berlin, Germany) · 2013Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
With recent advances in high-throughput proteomics and systems biology, there is a growing demand for new instruments that can precisely quantify a wide range of receptor-ligand binding kinetics in a high-throughput fashion. Here we demonstrate a surface plasmon resonance (SPR) imaging spectroscopy instrument capable of simultaneously extracting binding kinetics and affinities from 50 parallel microfluidic channels. The instrument utilizes large-area (~ cm(2)) metallic nanohole arrays as SPR sensing substrates and combines a broadband light source, a high-resolution imaging spectrometer and a low-noise CCD camera to extract spectral information from every channel in real time with a refractive index resolution of 7.7 × 10(-6) refractive index units. To demonstrate the utility of our instrument for quantifying a wide range of biomolecular interactions, each parallel microfluidic channel is coated with a biomimetic supported lipid membrane containing ganglioside (GM1) receptors. The binding kinetics of cholera toxin b (CTX-b) to GM1 are then measured in a single experiment from 50 channels. By combining the highly parallel microfluidic device with large-area periodic nanohole array chips, our SPR imaging spectrometer system enables high-throughput, label-free, real-time SPR biosensing, and its full-spectral imaging capability combined with nanohole arrays could enable integration of SPR imaging with concurrent surface-enhanced Raman spectroscopy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.