ArticleCell cycle (Georgetown, Tex.)2012
Heat shock induces apoptosis in human embryonic stem cells but a premature senescence phenotype in their differentiated progeny.
Article in Cell cycle (Georgetown, Tex.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- Mesenchymal Stem/Stromal Cells in Three-Dimensional Cell Culture: Ion Homeostasis and Ouabain-Induced Apoptosis.Biomedicines · 2023Article
- Monovalent ions and stress-induced senescence in human mesenchymal endometrial stem/stromal cells.Scientific reports · 2022Article
- Role of heat shock protein 60 in primed and naïve states of human pluripotent stem cells.PloS one · 2022Article
- Enhanced proliferation of rabbit chondrocytes by using a well circulated nanoshock system.Scientific reports · 2021Article
- Graphene-Induced Hyperthermia (GIHT) Combined With Radiotherapy Fosters Immunogenic Cell Death.Frontiers in oncology · 2021Article
- Heat Shock Alters Mesenchymal Stem Cell Identity and Induces Premature Senescence.Frontiers in cell and developmental biology · 2020Article
- Three-Dimensional Compaction Switches Stress Response Programs and Enhances Therapeutic Efficacy of Endometrial Mesenchymal Stem/Stromal Cells.Frontiers in cell and developmental biology · 2020Article
- Heat Shock Proteins and their Protective Roles in Stem Cell Biology.Stem cell reviews and reports · 2019Review
- Human Embryonic Stem Cells Acquire Responsiveness to TRAIL upon Exposure to Cisplatin.Stem cells international · 2019Article
- Chaperones and Beyond as Key Players in Pluripotency Maintenance.Frontiers in cell and developmental biology · 2019Review
- Article
- Selective Elimination of Human Induced Pluripotent Stem Cells Using Medium with High Concentration of L-Alanine.Scientific reports · 2018Article
- Human mesenchymal stem cells in spheroids improve fertility in model animals with damaged endometrium.Stem cell research & therapy · 2018Article
- Quiescent Human Mesenchymal Stem Cells Are More Resistant to Heat Stress than Cycling Cells.Stem cells international · 2018Article
- Redox environment in stem and differentiated cells: A quantitative approach.Redox biology · 2017Article
- Molecular Genetic Analysis of Human Endometrial Mesenchymal Stem Cells That Survived Sublethal Heat Shock.Stem cells international · 2017Article
- Effects of Aminoglycoside Antibiotics on Human Embryonic Stem Cell Viability during Differentiation In Vitro.Stem cells international · 2017Article
- Sublethal heat shock induces premature senescence rather than apoptosis in human mesenchymal stem cells.Cell stress & chaperones · 2014Article
- Human embryonic and induced pluripotent stem cells express TRAIL receptors and can be sensitized to TRAIL-induced apoptosis.Stem cells and development · 2013Article
- Lessons learned about human stem cell responses to ionizing radiation exposures: a long road still ahead of us.International journal of molecular sciences · 2013Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Embryonic stem cells (ESC) are able to self-renew and to differentiate into any cell type. To escape error transmission to future cell progeny, ESC require robust mechanisms to ensure genomic stability. It was stated that stress defense of mouse and human ESC against oxidative stress and irradiation is superior compared with differentiated cells. Here, we investigated heat shock response of human ESC (hESC) and their differentiated progeny. Fibroblast-like cells were generated by spontaneous hESC differentiation via embryoid bodies. Like normal human diploid fibroblasts, these cells have a finite lifespan in culture, undergo replicative senescence and die. We found that sublethal heat shock affected survival of both cell types, but in hESC it induced apoptosis, whereas in differentiated cells it produced cell cycle arrest and premature senescence phenotype. Heat shock survived hESC and differentiated cells restored the properties of initial cells. Heated hESC progeny exhibited pluripotent markers and the capacity to differentiate into the cells of three germ layers. Fibroblast-like cells resisted heat shock, proliferated for a limited number of passages and entered replicative senescence as unheated parental cells. Taken together, these results show for the first time that both hESC and their differentiated derivatives are sensitive to heat shock, but the mechanisms of their stress response are different: hESC undergo apoptosis, whereas differentiated cells under the same conditions exhibit stress-induced premature senescence (SIPS) phenotype. Both cell types that survived sublethal heat shock sustain parental cell properties.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.