ReviewCell cycle (Georgetown, Tex.)2012
End-on microtubule-dynein interactions and pulling-based positioning of microtubule organizing centers.
Review in Cell cycle (Georgetown, Tex.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 63 citations in OpenAlex.
- Centrosome migration and apical membrane formation during epithelial polarization in MDCK cysts.eLife · 2026Article
- Multiscale temporal tuning of force generation complex machinery governs cortical microtubule interactions during the first mitotic division inbioRxiv : the preprint server for biology · 2026Article
- Asynchronous mouse embryo polarization leads to heterogeneity in cell fate specification.eLife · 2025Article
- Modeling actin-microtubule crosstalk in migrating cells.Biophysical journal · 2025Article
- The GTP-tubulin cap is not the determinant of microtubule end stability in cells.Molecular biology of the cell · 2024Article
- Positioning centrioles and centrosomes.The Journal of cell biology · 2024Review
- Dynein localization and pronuclear movement in the C. elegans zygote.Cytoskeleton (Hoboken, N.J.) · 2022Article
- Reconstituting and Characterizing Actin-Microtubule Composites with Tunable Motor-Driven Dynamics and Mechanics.Journal of visualized experiments : JoVE · 2022Article
- Modeling reveals cortical dynein-dependent fluctuations in bipolar spindle length.Biophysical journal · 2021Article
- The coordination of spindle-positioning forces during the asymmetric division of the Caenorhabditis elegans zygote.EMBO reports · 2021Article
- Microtubule-dependent pushing forces contribute to long-distance aster movement and centration inMolecular biology of the cell · 2020Article
- A High-Throughput Cellular Screening Assay for Small-Molecule Inhibitors and Activators of Cytoplasmic Dynein-1-Based Cargo Transport.SLAS discovery : advancing life sciences R & D · 2020Article
- TANGLED1 mediates microtubule interactions that may promote division plane positioning in maize.The Journal of cell biology · 2020Article
- Mechanics of Multicentrosomal Clustering in Bipolar Mitotic Spindles.Biophysical journal · 2020Article
- Centering and Shifting of Centrosomes in Cells.Cells · 2020Review
- Poor old pores-The challenge of making and maintaining nuclear pore complexes in aging.The FEBS journal · 2020Review
- From isolated structures to continuous networks: A categorization of cytoskeleton-based motile engineered biological microstructures.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2019Review
- The Spindle: Integrating Architecture and Mechanics across Scales.Trends in cell biology · 2018Review
- Cell polarity: having and making sense of direction-on the evolutionary significance of the primary cilium/centrosome organ in Metazoa.Open biology · 2018Review
- Direct measurement of the strength of microtubule attachment to yeast centrosomes.Molecular biology of the cell · 2017Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During important cellular processes such as centrosome and spindle positioning, dynein at the cortex interacts with dynamic microtubules in an apparent "end-on" fashion. It is well-established that dynein can generate forces by moving laterally along the microtubule lattice, but much less is known about dynein's interaction with dynamic microtubule ends. In this paper, we review recent in vitro experiments that show that dynein, attached to an artificial cortex, is able to capture microtubule ends, regulate microtubule dynamics and mediate the generation of pulling forces on shrinking microtubules. We further review existing ideas on the involvement of dynein-mediated cortical pulling forces in the positioning of microtubule organizing centers such as centrosomes. Recent in vitro experiments have demonstrated that cortical pulling forces in combination with pushing forces can lead to reliable centering of microtubule asters in quasi two-dimensional microfabricated chambers. In these experiments, pushing leads to slipping of microtubule ends along the chamber boundaries, resulting in an anisotropic distribution of cortical microtubule contacts that favors centering, once pulling force generators become engaged. This effect is predicted to be strongly geometry-dependent, and we therefore finally discuss ongoing efforts to repeat these experiments in three-dimensional, spherical and deformable geometries.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.