ArticleJournal of clinical microbiology2012
Specific detection of enteroaggregative hemorrhagic Escherichia coli O104:H4 strains by use of the CRISPR locus as a target for a diagnostic real-time PCR.
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 75 citations in OpenAlex.
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- Genomic Markers Distinguishing Shiga Toxin-ProducingPathogens (Basel, Switzerland) · 2025Article
- Enhancing detection of STEC in the meat industry: insights into virulence of priority STEC.Frontiers in microbiology · 2025Article
- First report and genomic characterization of Escherichia coli O111:H12 serotype from raw mussels in Türkiye.BMC genomics · 2024Article
- A preliminary study of the use of MinION sequencing to specifically detect Shiga toxin-producing Escherichia coli in culture swipes containing multiple serovars of this species.Scientific reports · 2023Article
- CRISPR-Based Diagnostics and Microfluidics for COVID-19 Point-of-Care Testing: A Review of Main Applications.Molecular biotechnology · 2023Review
- Ten decadal advances in fungal biology leading towards human well-being.Fungal diversity · 2022Review
- Diversity of Potentially PathogenicMicroorganisms · 2021Article
- CRISPR-Based Diagnosis of Infectious and Noninfectious Diseases.Biological procedures online · 2020Review
- Utilization of Clustered Regularly Interspaced Short Palindromic Repeats to GenotypeFrontiers in microbiology · 2020Article
- Prevalence of Enteropathogens and Virulence Traits in Brazilian Children With and Without Diarrhea.Frontiers in cellular and infection microbiology · 2020Article
- Laboratory Methods in Molecular Epidemiology: Bacterial Infections.Microbiology spectrum · 2018Review
- Article
- Genetic Analysis of Virulence Potential ofFrontiers in microbiology · 2018Article
- Identification of Novel Biomarkers for Priority Serotypes of Shiga Toxin-ProducingFrontiers in microbiology · 2018Article
- Suppressing the CRISPR/Cas adaptive immune system in bacterial infections.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017Review
- Shiga Toxin-Producing Serogroup O91 Escherichia coli Strains Isolated from Food and Environmental Samples.Applied and environmental microbiology · 2017Article
- Draft Genome Sequences ofGenome announcements · 2017Article
- Public Health Research Resulting from One of the World's Largest Outbreaks Caused by Entero-HemorrhagicFrontiers in public health · 2017Review
- Escherichia coli O104 in Feedlot Cattle Feces: Prevalence, Isolation and Characterization.PloS one · 2016Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In 2011, a large outbreak of an unusual bacterial strain occurred in Europe. This strain was characterized as a hybrid of an enteroaggregative Escherichia coli (EAEC) and a Shiga toxin-producing E. coli (STEC) strain of the serotype O104:H4. Here, we present a single PCR targeting the clustered regularly interspaced short palindromic repeats locus of E. coli O104:H4 (CRISPR(O104:H4)) for specific detection of EAEC STEC O104:H4 strains from different geographical locations and time periods. The specificity of the CRISPR(O104:H4) PCR was investigated using 1,321 E. coli strains, including reference strains for E. coli O serogroups O1 to O186 and flagellar (H) types H1 to H56. The assay was compared for specificity using PCR assays targeting different O104 antigen-encoding genes (wbwC(O104), wzx(O104), and wzy(O104)). The PCR assays reacted with all types of E. coli O104 strains (O104:H2, O104:H4, O104:H7, and O104:H21) and with E. coli O8 and O9 strains carrying the K9 capsular antigen and were therefore not specific for detection of the EAEC STEC O104:H4 type. A single PCR developed for the CRISPR(O104:H4) target was sufficient for specific identification and detection of the 48 tested EAEC STEC O104:H4 strains. The 35 E. coli O104 strains expressing H types other than H4 as well as 8 E. coli strains carrying a K9 capsular antigen tested all negative for the CRISPR(O104:H4) locus. Only 12 (0.94%) of the 1,273 non-O104:H4 E. coli strains (serotypes Ont:H2, O43:H2, O141:H2, and O174:H2) reacted positive in the CRISPR(O104:H4) PCR (99.06% specificity).
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