Evidence map›Paper›PMID 22894715›Full record

ArticleASN neuro2012

Neuroblast migration and P2Y(1) receptor mediated calcium signalling depend on 9-O-acetyl GD3 ganglioside.

Marcelo F Santiago, Eliana Scemes

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ASN neuro, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact, top 84% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 11 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. ATP-induced CaCellular and molecular life sciences : CMLS · 2017
    Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 2 countries.

Marcelo F SantiagoInstituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Eliana Scemes
Albert Einstein College of Medicine · USUniversidade Federal do Rio de Janeiro · BR

Funding

Translational Neuroimaging CoreP30HD071593 · NICHD · ALBERT EINSTEIN COLLEGE OF MEDICINE, INC · PI WALKLEY, STEVEN UPSHAW · 2011 to 2015
$5.6M
Mechanisms of ATP Release from Glial ProgenitorsR01NS052245 · NINDS · ALBERT EINSTEIN COLLEGE OF MEDICINE · PI SCEMES, ELIANA · 2007 to 2011
$1.6M
NICHD NIH HHS 1P30HD071593-01NICHD NIH HHS P30 HD071593NINDS NIH HHS R01 NS052245NINDS NIH HHS R01-NS054225
6 · The paper itself

Abstract

Previous studies indicated that a ganglioside 9acGD3 (9-O-acetyl GD3) antibody [the J-Ab (Jones antibody)] reduces GCP (granule cell progenitor) migration in vitro and in vivo. We here investigated, using cerebellar explants of post-natal day (P) 6 mice, the mechanism by which 9acGD3 reduces GCP migration. We found that immunoblockade of the ganglioside with the J-Ab or the lack of GD3 synthase reduced GCP in vitro migration and the frequency of Ca(2+) oscillations. Immunocytochemistry and pharmacological assays indicated that GCPs expressed P2Y(1)Rs (P2Y(1) receptors) and that deletion or blockade of these receptors decreased the migration rate of GCPs and the frequency of Ca(2+) oscillations. The reduction in P2Y(1)-mediated calcium signals seen in Jones-treated and GD3 synthase-null GCPs were paralleled by P2Y(1)R internalization. We conclude that 9acGD3 controls GCP migration by influencing P2Y(1)R cellular distribution and function.

Indexed as

Adenosine DiphosphateAdenosine TriphosphateAnimalsAnimals, NewbornAntibodiesCalciumCalcium SignalingCell MovementCerebellumGangliosidesGene Expression Regulation, DevelopmentalGlial Fibrillary Acidic ProteinGreen Fluorescent ProteinsMiceMice, KnockoutMicrotubule-Associated Proteins3'-O-(4-benzoyl)benzoyladenosine 5'-triphosphate9-O-acetyl-GD3 gangliosideAdenosine DiphosphateAdenosine TriphosphateAntibodiesCalciumenhanced green fluorescent proteinganglioside A2B5ganglioside, GD3GangliosidesGlial Fibrillary Acidic ProteinGreen Fluorescent ProteinsMicrotubule-Associated ProteinsMtap2 protein, mouseN(6)-methyl-2'-deoxyadenosine 3',5'-diphosphatePurinergic P2Y Receptor AgonistsPurinergic P2Y Receptor AntagonistsReceptors, Purinergic P2Y1Tubulin

Identifiers

PMID22894715
PMCPMC3442489
OpenAlexW2142522067

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.