ArticleAnalytical chemistry2012
Direct detection of adenosine in undiluted serum using a luminescent aptamer sensor attached to a terbium complex.
Article in Analytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Matrix-Activated Aptamer Sensors for Human Serum Analysis.Angewandte Chemie (International ed. in English) · 2026Article
- Capture-SELEX of DNA Aptamers for Highly Selective Binding of Folate.Chembiochem : a European journal of chemical biology · 2026Article
- A dual signal amplification strategy of Au@ZIF67 catalysis and target cycle amplification for sensitive chemiluminescence detection of adenosine.Mikrochimica acta · 2025Article
- Fluorescence Sensor for Zearalenone Detection Based on Oxidized Single-walled Carbon Nanohorns/N-doped Carbon Quantum Dots-aptamer.Journal of fluorescence · 2024Article
- Time-resolved Fluorescence DNA-based Sensors for Reducing Background Fluorescence of Environment.Journal of fluorescence · 2023Review
- Emerging Biosensing Methods to Monitor Lung Cancer Biomarkers in Biological Samples: A Comprehensive Review.Cancers · 2023Review
- Article
- Directed evolution and selection of biostable L-DNA aptamers with a mirror-image DNA polymerase.Nature biotechnology · 2022Article
- Overcoming Major Barriers to Developing Successful Sensors for Practical Applications Using Functional Nucleic Acids.Annual review of analytical chemistry (Palo Alto, Calif.) · 2022Review
- One-Step Homogeneous Immunoassay for the Detection of Influenza Virus Using Switching Peptide and Graphene Quencher.Biochip journal · 2022Article
- A photo-regulated aptamer sensor for spatiotemporally controlled monitoring of ATP in the mitochondria of living cells.Chemical science · 2019Article
- Label-Free Fluorescent Aptasensor for Small Targets via Displacement of Groove Bound Curcumin Molecules.Sensors (Basel, Switzerland) · 2019Article
- Colorimetric adenosine aptasensor based on DNA cycling amplification and salt-induced aggregation of gold nanoparticles.Mikrochimica acta · 2018Article
- New insights into a classic aptamer: binding sites, cooperativity and more sensitive adenosine detection.Nucleic acids research · 2017Article
- Efficient route to pre-organized and linear polyaminopolycarboxylates: Cy-TTHA, Cy-DTPA and mono/di- reactive,Tetrahedron letters · 2017Article
- Design of a synthetic luminescent probe from a biomolecule binding domain: selective detection of AU-rich mRNA sequences.Chemical science · 2017Article
- Electrochemical aptasensor for lung cancer-related protein detection in crude blood plasma samples.Scientific reports · 2016Article
- Development of a luminescent G-quadruplex-selective iridium(III) complex for the label-free detection of adenosine.Scientific reports · 2016Article
- A rapid enzymatic assay for high-throughput screening of adenosine-producing strains.Microbial biotechnology · 2015Article
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Authors and funding
4 authors.
Funding
Abstract
Aptamers, single-stranded nucleic acids that can selectively bind to various target molecules, have been widely used for constructing biosensors. A major challenge in this field, however, is direct sensing of analytes in complex biological media such as undiluted serum. While progress has been made in developing an inhomogeneous assay by using a preseparation step to wash away the interferences within serum, a facile strategy for direct detection of targets in homogeneous unprocessed serum is highly desired. We herein report a turn-on luminescent aptamer biosensor for the direct detection of adenosine in undiluted and unprocessed serum, by taking advantage of a terbium chelate complex with long luminescence lifetime to achieve time-resolved detection. The sensor exhibits a detection limit of 60 μM adenosine while marinating excellent selectivity that is comparable to those in buffer. The approach demonstrated here can be applied for direct detection and quantification of a broad range of analytes in biological media by using other aptamers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.