ArticleJournal of biomedical optics2012
Analysis of second-harmonic-generation microscopy in a mouse model of ovarian carcinoma.
Article in Journal of biomedical optics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- A Perspective Review: Analyzing Collagen Alterations in Ovarian Cancer by High-Resolution Optical Microscopy.Cancers · 2024Review
- On the quantitative analysis of lamellar collagen arrangement with second-harmonic generation imaging.Biomedical optics express · 2024Article
- Squeezing the eggs to grow: The mechanobiology of mammalian folliculogenesis.Frontiers in cell and developmental biology · 2022Review
- Multiscale anisotropy analysis of second-harmonic generation collagen imaging of mouse skin.Journal of biomedical optics · 2021Article
- Harmonic Generation Microscopy 2.0: New Tricks Empowering Intravital Imaging for Neuroscience.Frontiers in molecular biosciences · 2019Review
- Stromal alterations in ovarian cancers via wavelength dependent Second Harmonic Generation microscopy and optical scattering.BMC cancer · 2017Article
- Epithelial Ovarian Cancer Diagnosis of Second-Harmonic Generation Images: A Semiautomatic Collagen Fibers Quantification Protocol.Cancer informatics · 2017Article
- A Novel Intravital Imaging Window for Longitudinal Microscopy of the Mouse Ovary.Scientific reports · 2015Article
- Applications of second-harmonic generation imaging microscopy in ovarian and breast cancer.Perspectives in medicinal chemistry · 2015Article
- Diagnostic potential of multimodal imaging of ovarian tissue using optical coherence tomography and second-harmonic generation microscopy.Journal of medical imaging (Bellingham, Wash.) · 2014Article
- Simultaneous multiplane imaging of human ovarian cancer by volume holographic imaging.Journal of biomedical optics · 2014Article
- Nonlinear optical microscopy signal processing strategies in cancer.Cancer informatics · 2014Review
- Computational segmentation of collagen fibers from second-harmonic generation images of breast cancer.Journal of biomedical optics · 2014Article
- In vivo time-serial multi-modality optical imaging in a mouse model of ovarian tumorigenesis.Cancer biology & therapy · 2014Article
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Authors and funding
9 authors.
Funding
Abstract
Second-harmonic-generation (SHG) imaging of mouse ovaries ex vivo was used to detect collagen structure changes accompanying ovarian cancer development. Dosing with 4-vinylcyclohexene diepoxide and 7,12-dimethylbenz[a]anthracene resulted in histologically confirmed cases of normal, benign abnormality, dysplasia, and carcinoma. Parameters for each SHG image were calculated using the Fourier transform matrix and gray-level co-occurrence matrix (GLCM). Cancer versus normal and cancer versus all other diagnoses showed the greatest separation using the parameters derived from power in the highest-frequency region and GLCM energy. Mixed effects models showed that these parameters were significantly different between cancer and normal (P<0.008). Images were classified with a support vector machine, using 25% of the data for training and 75% for testing. Utilizing all images with signal greater than the noise level, cancer versus not-cancer specimens were classified with 81.2% sensitivity and 80.0% specificity, and cancer versus normal specimens were classified with 77.8% sensitivity and 79.3% specificity. Utilizing only images with greater than of 75% of the field of view containing signal improved sensitivity and specificity for cancer versus normal to 81.5% and 81.1%. These results suggest that using SHG to visualize collagen structure in ovaries could help with early cancer detection.
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