ArticleLab on a chip2012
An integrated microfluidic platform for in situ cellular cytokine secretion immunophenotyping.
Article in Lab on a chip, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 66 citations in OpenAlex.
- Progress in Organ Bioprinting for Regenerative Medicine.Engineering (Beijing, China) · 2024Article
- Microphysiological Systems for Cancer Immunotherapy Research and Development.Advanced biology · 2024Review
- Deciphering cellular complexity: advances and future directions in single-cell protein analysis.Frontiers in bioengineering and biotechnology · 2024Review
- Recent advances for cancer detection and treatment by microfluidic technology, review and update.Biological procedures online · 2022Review
- The Intriguing Landscape of Single-Cell Protein Analysis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Pancreas-on-a-Chip Technology for Transplantation Applications.Current diabetes reports · 2020Review
- Microfluidic Viscometer Using a Suspending Micromembrane for Measurement of Biosamples.Micromachines · 2020Article
- Human stroma and epithelium co-culture in a microfluidic model of a human prostate gland.Biomicrofluidics · 2019Article
- Mass-producible microporous silicon membranes for specific leukocyte subset isolation, immunophenotyping, and personalized immunomodulatory drug screening in vitro.Lab on a chip · 2019Article
- Mobile Microfluidics.Bioengineering (Basel, Switzerland) · 2019Review
- An integrated adipose-tissue-on-chip nanoplasmonic biosensing platform for investigating obesity-associated inflammation.Lab on a chip · 2018Article
- Centrifugal microfluidics for sorting immune cells from whole blood.Sensors and actuators. B, Chemical · 2017Article
- Surface micromachining of polydimethylsiloxane for microfluidics applications.Biomicrofluidics · 2016Article
- Multi-Dimensional Nanostructures for Microfluidic Screening of Biomarkers: From Molecular Separation to Cancer Cell Detection.Annals of biomedical engineering · 2016Review
- Microfluidics: A new tool for modeling cancer-immune interactions.Trends in cancer · 2016Article
- Microfluidic Organ/Body-on-a-Chip Devices at the Convergence of Biology and Microengineering.Sensors (Basel, Switzerland) · 2015Review
- Label-free cytokine micro- and nano-biosensing towards personalized medicine of systemic inflammatory disorders.Advanced drug delivery reviews · 2015Review
- Reconfigurable microfluidic device with integrated antibody arrays for capture, multiplexed stimulation, and cytokine profiling of human monocytes.Biomicrofluidics · 2015Article
- Rapid, automated, parallel quantitative immunoassays using highly integrated microfluidics and AlphaLISA.Scientific reports · 2015Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Rapid, quantitative detection of cell-secreted biomarker proteins with a low sample volume holds great promise to advance cellular immunophenotyping techniques for personalized diagnosis and treatment of infectious diseases. Here we achieved such an assay with the THP-1 human acute moncytic leukemia cell line (a model for human monocyte) using a highly integrated microfluidic platform incorporating a no-wash bead-based chemiluminescence immunodetection scheme. Our microfluidic device allowed us to stimulate cells with lipopolysaccharide (LPS), which is an endotoxin causing septic shock due to severely pronounced immune response of the human body, under a well-controlled on-chip environment. Tumor necrosis factor-alpha (TNF-α) secreted from stimulated THP-1 cells was subsequently measured within the device with no flushing process required. Our study achieved high-sensitivity cellular immunophenotyping with 20-fold fewer cells than current cell-stimulation assay. The total assay time was also 7 times shorter than that of a conventional enzyme-linked immunosorbent assay (ELISA). Our strategy of monitoring immune cell functions in situ using a microfluidic platform could impact future medical treatments of acute infectious diseases and immune disorders by enabling a rapid, sample-efficient cellular immunophenotyping analysis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.