ArticleHistochemistry and cell biology2013
Label-free detection of peripheral nerve tissues against adjacent tissues by spontaneous Raman microspectroscopy.
Article in Histochemistry and cell biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 37 citations in OpenAlex.
- Rice straw sample preparation protocol for cell wall major constituent analysis via Raman spectroscopy.STAR protocols · 2026Article
- Spatially multiplexed random-access Raman probe for in vivo tissue measurements.Biomedical optics express · 2026Article
- Spectral Region Optimization and Machine Learning-Based Nonlinear Spectral Analysis for Raman Detection of Cardiac Fibrosis Following Myocardial Infarction.International journal of molecular sciences · 2025Article
- Multimodal Optical Imaging Combined with Radiomic Analysis for Fibrotic Cardiac Tissue Investigation.Analytical chemistry · 2025Article
- Biochemical component analysis of human myopic corneal stroma using the Raman spectrum.International ophthalmology · 2024Article
- Raman difference spectroscopy and U-Net convolutional neural network for molecular analysis of cutaneous neurofibroma.PloS one · 2024Article
- High-throughput line-illumination Raman microscopy with multislit detection.Biomedical optics express · 2023Article
- High-sensitivity hyperspectral vibrational imaging of heart tissues by mid-infrared photothermal microscopy.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2022Article
- Automated non-invasive identification of pelvic autonomic nerves with a handheld Raman spectrometer and potential application to nerve-sparing colorectal surgery: a preliminary study in surgical specimens.Translational cancer research · 2021Article
- Label-free Evaluation of Myocardial Infarct in Surgically Excised Ventricular Myocardium by Raman Spectroscopy.Scientific reports · 2018Article
- Label-free Molecular Imaging and Analysis by Raman Spectroscopy.Acta histochemica et cytochemica · 2018Article
- Coherent anti-Stokes Raman scattering rigid endoscope toward robot-assisted surgery.Biomedical optics express · 2018Article
- Rapid and accurate peripheral nerve imaging by multipoint Raman spectroscopy.Scientific reports · 2017Article
- Using Raman spectroscopy to characterize biological materials.Nature protocols · 2016Article
- Ex vivo peripheral nerve detection of rats by spontaneous Raman spectroscopy.Scientific reports · 2015Article
- Direct label-free measurement of the distribution of small molecular weight compound inside thick biological tissue using coherent Raman microspectroscopy.Scientific reports · 2015Article
- The Histochem Cell Biol conspectus: the year 2013 in review.Histochemistry and cell biology · 2014Review
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Detection of peripheral nerve tissues during surgery is required to avoid neural disturbance following surgery as an aspect of realizing better functional outcome. We provide a proof-of-principle demonstration of a label-free detection technique of peripheral nerve tissues, including myelinated and unmyelinated nerves, against adjacent tissues that employ spontaneous Raman microspectroscopy. To investigate the Raman spectral features of peripheral nerves in detail, we used unfixed sectioned samples. Raman spectra of myelinated nerve, unmyelinated nerve, fibrous connective tissue, skeletal muscle, tunica media of blood vessel, and adipose tissue of Wistar rats were analyzed, and Raman images of the tissue distribution were constructed using the map of the ordinary least squares regression (OLSR) estimates. We found that nerve tissues exhibited a specific Raman spectrum arising from axon or myelin sheath, and that the nerve tissues can be selectively detected against the other tissues. Moreover, myelinated and unmyelinated nerves can be distinguished by the intensity differences of 2,855 cm⁻¹, and 2,945 cm⁻¹, which are mainly derived from lipid and protein contents of nerve fibers. We applied this method to unfixed section samples of human periprostatic tissues excised from prostatic cancer patients. Myelinated nerves, unmyelinated nerves, fibrous connective tissues, and adipose tissues of the periprostatic tissues were separately detected by OLSR analysis. These results suggest the potential of the Raman spectroscopic observation for noninvasive and label-free nerve detection, and we expect this method could be a key technique for nerve-sparing surgery.
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