ArticleBioengineered
Engineering of N-acetylglucosamine metabolism for improved antibiotic production in Streptomyces coelicolor A3(2) and an unsuspected role of NagA in glucosamine metabolism.
Article in Bioengineered. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 47 citations in OpenAlex.
- When, where, and why specialised metabolites are produced: inferring function from expression control.Essays in biochemistry · 2026Review
- The growth and pathogenesis ofInfection and immunity · 2026Article
- The novel GlcNAc 6-phosphate dehydratase NagS governs a metabolic checkpoint that controls nutrient signaling in Streptomyces.PLoS biology · 2025Article
- Polyester degradation by soil bacteria: identification of conserved BHETase enzymes in Streptomyces.Communications biology · 2024Article
- Allosteric regulation by c-di-AMP modulates a complete N-acetylglucosamine signaling cascade in Saccharopolyspora erythraea.Nature communications · 2024Article
- Systems-wide analysis of the ROK-family regulatory geneApplied and environmental microbiology · 2023Article
- Lunaemycins, New Cyclic Hexapeptide Antibiotics from the Cave Moonmilk-DwellerInternational journal of molecular sciences · 2023Article
- Article
- Diversity and prevalence of ANTAR RNAs across actinobacteria.BMC microbiology · 2021Article
- Article
- Significant release and microbial utilization of amino sugars and D-amino acid enantiomers from microbial cell wall decomposition in soils.Soil biology & biochemistry · 2018Article
- GntR Family Regulator DasR Controls Acetate Assimilation by Directly Repressing theJournal of bacteriology · 2018Article
- Structural and functional determination of homologs of theThe Journal of biological chemistry · 2018Article
- A Phenotypic and Genotypic Analysis of the Antimicrobial Potential of CultivableFrontiers in microbiology · 2016Article
- Article
- Toward a new focus in antibiotic and drug discovery from the Streptomyces arsenal.Frontiers in microbiology · 2015Review
- Triggers and cues that activate antibiotic production by actinomycetes.Journal of industrial microbiology & biotechnology · 2014Review
- The ROK family regulator Rok7B7 pleiotropically affects xylose utilization, carbon catabolite repression, and antibiotic production in streptomyces coelicolor.Journal of bacteriology · 2013Article
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Authors and funding
5 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
N-acetylglucosamine (GlcNAc), the monomer of chitin and constituent of bacterial peptidoglycan, is a preferred carbon and nitrogen source for streptomycetes. Recent studies have revealed new functions of GlcNAc in nutrient signaling of bacteria. Exposure to GlcNAc activates development and antibiotic production of Streptomyces coelicolor under poor growth conditions (famine) and blocks these processes under rich conditions (feast). Glucosamine-6-phosphate (GlcN-6P) is a key molecule in this signaling pathway and acts as an allosteric effector of a pleiotropic transcriptional repressor DasR, the regulon of which includes the GlcNAc metabolic enzymes N-actetylglucosamine-6-phosphate (GlcNAc-6P) deacetylase (NagA) and GlcN-6P deaminase (NagB). Intracellular accumulation of GlcNAc-6P and GlcN-6P enhanced production of the pigmented antibiotic actinorhodin. When the nagB mutant was challenged with GlcNAc or GlcN, spontaneous second-site mutations that relieved the toxicity of the accumulated sugar phosphates were obtained. Surprisingly, deletion of nagA also relieved toxicity of GlcN, indicating novel linkage between the GlcN and GlcNAc utilization pathways. The strongly enhanced antibiotic production observed for many suppressor mutants shows the potential of the modulation of GlcNAc and GlcN metabolism as a metabolic engineering tool toward the improvement of antibiotic productivity or even the discovery of novel compounds.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.