ArticleCellular microbiology2012
Identification of a cis-acting DNA-protein interaction implicated in singular var gene choice in Plasmodium falciparum.
Article in Cellular microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- ThemSphere · 2024Article
- The human malaria parasiteProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Rapid activation of distinct members of multigene families in Plasmodium spp.Communications biology · 2020Article
- Independent regulation of Plasmodium falciparum rif gene promoters.Scientific reports · 2018Article
- Chromatin Accessibility-Based Characterization of the Gene Regulatory Network Underlying Plasmodium falciparum Blood-Stage Development.Cell host & microbe · 2018Article
- Malaria Epigenetics.Cold Spring Harbor perspectives in medicine · 2017Review
- Knockdown of the Plasmodium falciparum SURFIN4.1 antigen leads to an increase of its cognate transcript.PloS one · 2017Article
- Trans-acting GC-rich non-coding RNA at var expression site modulates gene counting in malaria parasite.Nucleic acids research · 2016Article
- Recent advances in malaria genomics and epigenomics.Genome medicine · 2016Review
- Epigenetic Regulation of Virulence Gene Expression in Parasitic Protozoa.Cell host & microbe · 2016Review
- Deciphering the principles that govern mutually exclusive expression of Plasmodium falciparum clag3 genes.Nucleic acids research · 2015Article
- Transcription of the var genes from a freshly-obtained field isolate of Plasmodium falciparum shows more variable switching patterns than long laboratory-adapted isolates.Malaria journal · 2015Article
- A var gene upstream element controls protein synthesis at the level of translation initiation in Plasmodium falciparum.PloS one · 2014Article
- Silence, activate, poise and switch! Mechanisms of antigenic variation in Plasmodium falciparum.Cellular microbiology · 2013Review
- Analysis of the spatial and temporal arrangement of transcripts over intergenic regions in the human malarial parasite Plasmodium falciparum.BMC genomics · 2013Article
- Insulator-like pairing elements regulate silencing and mutually exclusive expression in the malaria parasite Plasmodium falciparum.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plasmodium falciparum is responsible for the most severe form of malaria in humans. Antigenic variation of P. falciparum erythrocyte membrane protein 1 leads to immune evasion and occurs through switches in mutually exclusive var gene transcription. The recent progress in Plasmodium epigenetics notwithstanding, the mechanisms by which singularity of var activation is achieved are unknown. Here, we employed a functional approach to dissect the role of var gene upstream regions in mutually exclusive activation. Besides identifying sequence elements involved in activation and initiation of transcription, we mapped a region downstream of the transcriptional start site that is required to maintain singular var gene choice. Activation of promoters lacking this sequence occurs no longer in competition with endogenous var genes. Within this region we pinpointed a sequence-specific DNA-protein interaction involving a cis-acting sequence motif that is conserved in the majority of var loci. These results suggest an important role for this interaction in mutually exclusive locus recognition. Our findings are furthermore consistent with a novel mechanism for the control of singular gene choice in eukaryotes. In addition to their importance in P. falciparum antigenic variation, our results may also help to explain similar processes in other systems.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.