ArticleThe Journal of biological chemistry2012
Lanthionine synthetase C-like protein 1 interacts with and inhibits cystathionine β-synthase: a target for neuronal antioxidant defense.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 49 citations in OpenAlex.
- Experimental upregulation of Lancl1 promotes axon regeneration after optic nerve injury in vivo.Experimental neurology · 2026Article
- Loss of LanC-like proteins delays post-injury regeneration of aging skeletal muscles.bioRxiv : the preprint server for biology · 2026Article
- Oxidative Stress and Iron Addiction: A Comparative Study of 1321N1 Astrocytoma and T98G Glioblastoma Cells with Differential Expression of L-Cysteine-Metabolizing Enzymes.Biomolecules · 2025Article
- Ligustilide-loaded liposome ameliorates mitochondrial impairments and improves cognitive function via the PKA/AKAP1 signaling pathway in a mouse model of Alzheimer's disease.CNS neuroscience & therapeutics · 2024Article
- Article
- Ferulic Acid Protects Human Lens Epithelial Cells against Ionizing Radiation-Induced Oxidative Damage by Activating Nrf2/HO-1 Signal Pathway.Oxidative medicine and cellular longevity · 2022Article
- Identification of Monoallelically Expressed Genes Associated with Economic Traits in Hanwoo (Korean Native Cattle).Animals : an open access journal from MDPI · 2021Article
- Biological Effects of Hydrogen Sulfide and Its Protective Role in Intracerebral Hemorrhage.Journal of molecular neuroscience : MN · 2020Review
- Cystathionine β-synthase is involved in cysteine biosynthesis and HScientific reports · 2020Article
- An overview of sulfur-containing compounds originating from natural metabolites: Lanthionine ketimine and its analogues.Analytical biochemistry · 2020Review
- Review
- LanCL proteins are not Involved in Lanthionine Synthesis in Mammals.Scientific reports · 2017Article
- Review
- Huntingtin-Interacting Protein 1-Related Protein Plays a Critical Role in Dendritic Development and Excitatory Synapse Formation in Hippocampal Neurons.Frontiers in molecular neuroscience · 2017Article
- Article
- Expanded natural product diversity revealed by analysis of lanthipeptide-like gene clusters in actinobacteria.Applied and environmental microbiology · 2015Article
- MPTP's pathway of toxicity indicates central role of transcription factor SP1.Archives of toxicology · 2015Article
- Alternative functions of the brain transsulfuration pathway represent an underappreciated aspect of brain redox biochemistry with significant potential for therapeutic engagement.Free radical biology & medicine · 2015Review
- Structure and mechanism of lanthipeptide biosynthetic enzymes.Current opinion in structural biology · 2014Review
- Developmental and activity-dependent expression of LanCL1 confers antioxidant activity required for neuronal survival.Developmental cell · 2014Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The finding that eukaryotic lanthionine synthetase C-like protein 1 (LanCL1) is a glutathione-binding protein prompted us to investigate the potential relationship between LanCL1 and cystathionine β-synthase (CBS). CBS is a trans-sulfuration enzyme critical for the reduced glutathione (GSH) synthesis and GSH-dependent defense against oxidative stress. In this study we found that LanCL1 bound to CBS in mouse cortex and HEK293 cells. Mapping studies revealed that the binding region in LanCL1 spans amino acids 158-169, and that in CBS contains N-terminal and C-terminal regulatory domains. Recombinant His-LanCL1 directly bound endogenous CBS from mouse cortical lysates and inhibited its activity. Overexpression of LanCL1 inhibited CBS activity in HEK293 cells. CBS activity is reported to be regulated by oxidative stress. Here we found that oxidative stress induced by H(2)O(2) or glutamate lowered the GSH/GSSG ratio, dissociated LanCL1 from CBS, and elevated CBS activity in primary rat cortical neurons. Decreasing the GSH/GSSG ratio by adding GSSG to cellular extracts also dissociated LanCL1 from CBS. Either lentiviral knockdown of LanCL1 or specific disruption of the LanCL1-CBS interaction using the peptide Tat-LanCL1(153-173) released CBS activity in neurons but occluded CBS activation in response to oxidative stress, indicating the major contribution of the LanCL1-CBS interaction to the regulation of CBS activity. Furthermore, LanCL1 knockdown or Tat-LanCL1(153-173) treatment reduced H(2)O(2) or glutamate-induced neuronal damage. This study implies potential therapeutic value in targeting the LanCL1-CBS interaction for neuronal oxidative stress-related diseases.
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