ArticleProteomics2012
Validation of regulated protein phosphorylation events in yeast by quantitative mass spectrometry analysis of purified proteins.
Article in Proteomics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 31 citations in OpenAlex.
- Decoding the function of Atg13 phosphorylation reveals a role of Atg11 in bulk autophagy initiation.EMBO reports · 2024Article
- New biomarkers underlying acetic acid tolerance in the probiotic yeast Saccharomyces cerevisiae var. boulardii.Applied microbiology and biotechnology · 2024Article
- A Causal Model of Ion Interference Enables Assessment and Correction of Ratio Compression in Multiplex Proteomics.Molecular & cellular proteomics : MCP · 2024Article
- Chemical Acetylation of Ligands and Two-Step Digestion Protocol for Reducing Codigestion in Affinity Purification-Mass Spectrometry.Journal of proteome research · 2023Article
- Post-Translational Modifications of Histones Are Versatile Regulators of Fungal Development and Secondary Metabolism.Toxins · 2022Review
- A phosphatase-centric mechanism drives stress signaling response.EMBO reports · 2021Article
- Regulation of Pkc1 Hyper-Phosphorylation by Genotoxic Stress.Journal of fungi (Basel, Switzerland) · 2021Article
- Peroxiredoxin promotes longevity and HeLife · 2020Article
- Mechanical stress impairs pheromone signaling via Pkc1-mediated regulation of the MAPK scaffold Ste5.The Journal of cell biology · 2019Article
- Novel interconnections of HOG signaling revealed by combined use of two proteomic software packages.Cell communication and signaling : CCS · 2019Article
- CDK and MAPK Synergistically Regulate Signaling Dynamics via a Shared Multi-site Phosphorylation Region on the Scaffold Protein Ste5.Molecular cell · 2018Article
- Light-sensing via hydrogen peroxide and a peroxiredoxin.Nature communications · 2017Article
- Identifying protein kinase-specific effectors of the osmostress response in yeast.Science signaling · 2017Article
- The Late S-Phase Transcription Factor Hcm1 Is Regulated through Phosphorylation by the Cell Wall Integrity Checkpoint.Molecular and cellular biology · 2016Article
- Discordance between protein and transcript levels detected by selected reaction monitoring.Plant signaling & behavior · 2015Article
- Hrr25 kinase promotes selective autophagy by phosphorylating the cargo receptor Atg19.EMBO reports · 2014Article
- Rewiring yeast osmostress signalling through the MAPK network reveals essential and non-essential roles of Hog1 in osmoadaptation.Scientific reports · 2014Article
- Early steps in autophagy depend on direct phosphorylation of Atg9 by the Atg1 kinase.Molecular cell · 2014Article
- Genotoxic stress prevents Ndd1-dependent transcriptional activation of G2/M-specific genes in Saccharomyces cerevisiae.Molecular and cellular biology · 2014Article
- MAPK Hog1 closes the S. cerevisiae glycerol channel Fps1 by phosphorylating and displacing its positive regulators.Genes & development · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 5 institutions in 3 countries.
Funding
Abstract
Global phosphoproteomic studies based on MS have generated qualitative and quantitative data describing protein phosphorylation events in various biological systems. Since high-throughput data for protein modifications are inherently incomplete, we developed a strategy to extend and validate such primary datasets. We selected interesting protein candidates from a global screen in yeast and employed a modified histidine biotin tag that allows tandem affinity purifications of our targets under denaturing conditions. Products in question can be digested directly from affinity resins and phosphopeptides can be further enriched via TiO(2) before MS analysis. Our robust protocol can be amended for SILAC as well as iTRAQ quantifications or label-free approaches based on selective reaction monitoring, allowing completion of the phosphorylation pattern in a first step, followed by a detailed analysis of the phosphorylation kinetics. We exemplify the value of such a strategy by an in-depth analysis of Pan1, a highly phosphorylated factor involved in early steps of endocytosis. The study of Pan1 under osmotic stress conditions in different mutant backgrounds allowed us to differentiate between mitogen-activated protein kinase Hog1 driven and Hog1 independent stress responses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.