ArticleDevelopment genes and evolution2012
Heat shock-mediated misexpression of genes in the beetle Tribolium castaneum.
Article in Development genes and evolution, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 80 citations in OpenAlex.
- Characterization of Heat Shock Protein Expression and Its Application to Temporal Gene Expression in the Nematode Pristionchus pacificus.Development, growth & differentiation · 2026Article
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- Temporal control of RNAi reveals both robust and labile feedback loops in the segmentation clock of the red flour beetle.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Next generation marker-based vector concepts for rapid and unambiguous identification of single and double homozygous transgenic organisms.Biology open · 2023Article
- Phenotypic screen and transcriptomics approach complement each other in functional genomics of defensive stink gland physiology.BMC genomics · 2022Article
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- Mutually Exclusive Expression of Closely Related Odorant-Binding Proteins 9A and 9B in the Antenna of the Red Flour BeetleBiomolecules · 2021Article
- The βScientific reports · 2021Article
- Fertility and mortality impacts of thermal stress from experimental heatwaves on different life stages and their recovery in a model insect.Royal Society open science · 2021Article
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- An ancestral apical brain region contributes to the central complex under the control ofeLife · 2019Article
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- A key role forDevelopment (Cambridge, England) · 2017Article
- Evolution and multiple roles of the Pancrustacea specific transcription factor zelda in insects.PLoS genetics · 2017Article
- Progress and Prospects of CRISPR/Cas Systems in Insects and Other Arthropods.Frontiers in physiology · 2017Review
- Morphological and Transcriptomic Analysis of a Beetle Chemosensory System Reveals a Gnathal Olfactory Center.BMC biology · 2016Article
- The insect central complex as model for heterochronic brain development-background, concepts, and tools.Development genes and evolution · 2016Review
- Live imaging of Tribolium castaneum embryonic development using light-sheet-based fluorescence microscopy.Nature protocols · 2015Article
- Large scale RNAi screen in Tribolium reveals novel target genes for pest control and the proteasome as prime target.BMC genomics · 2015Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Insect gene function has mainly been studied in the fruit fly Drosophila melanogaster because in this species many techniques and resources are available for gene knock down and the ectopic activation of gene function. However, in order to study biological aspects that are not represented by the Drosophila model, and in order to test to what degree gene functions are conserved within insects and what changes in gene function accompanied the evolution of novel traits, the establishment of respective tools in other insect species is required. While gene knock down can be induced by RNA interference in many insects, methods to misexpress genes are much less developed. In order to allow misexpression of genes in a timely controlled manner in the red flour beetle Tribolium castaneum, we have established a heat shock-mediated misexpression system. We show that endogenous heat shock elements perform better than artificial heat shock elements derived from vertebrates. We carefully determine the optimal conditions for heat shock and define a core promoter for use in future constructs. Finally, using this system, we study the effects of misexpressing the head patterning gene Tc-orthodenticle1 (Tc-otd1), We show that Tc-otd1 suppresses Tc-wingless (Tc-wg) in the trunk and to some degree in the head.
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